Li Li, Xie Xiangxue, Zhao Guoqiang, He Jiajun, Zhang Yongliang
Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
Ruminant Product Research and Development Department, Guangdong VTR Bio-Tech Co., Ltd., Zhuhai, China.
Front Plant Sci. 2024 Sep 25;15:1441873. doi: 10.3389/fpls.2024.1441873. eCollection 2024.
The purpose of this experiment was to explore the effect of adding cellulase and laccase on fermentation quality and microbial community in mixed silage of corn stover and wet brewer's grains. Try to a new approach for the proper preservation and utilization of the agro-industrial by-products (corn stover and wet brewer's grains).
The experiment was divided into four groups: CK (control), C (cellulase, 120 U/g fresh matter [FM]), L (laccase, 50 U/g FM), CL (cellulase 120 U/g FW and laccase 50 U/g FM), and the chemical composition, fermentation quality, microbial population and microbial community in mixed silage of corn stover and wet brewer's grains after 30 day's fermentation were determined.
Compared to control, the addition of cellulase significantly increased crude protein (CP), water-soluble carbohydrate (WSC), lactic acid bacteria (LAB) counts, while significantly decreased the neutral detergent fiber (NDF), acid detergent fiber (ADF) content ( < 0.05). Adding laccase significantly decreased the acid detergent lignin (ADL) content ( < 0.05). Combined application of cellulase and laccase significantly increased the CP, WSC content and LAB counts, while significantly decreased pH value, NDF, ADF and ADL content ( < 0.05), thereby improving fermentation quality. In addition, the application of cellulase and laccase increased the abundance of Firmicutes and LAB genera, and decreased microbial diversity level of the sample.
The combined application of cellulase and laccase further improved fermentation quality and microbial community in mixed silage of corn stover and wet brewer's grains.
本试验旨在探究添加纤维素酶和漆酶对玉米秸秆与啤酒糟混合青贮发酵品质及微生物群落的影响。尝试为农业副产品（玉米秸秆和啤酒糟）的妥善保存与利用探索新方法。
试验分为四组：CK（对照组）、C（纤维素酶，120 U/g鲜物质[FM]）、L（漆酶，50 U/g FM）、CL（纤维素酶120 U/g FW和漆酶50 U/g FM），测定玉米秸秆与啤酒糟混合青贮30天后的化学成分、发酵品质、微生物数量及微生物群落。
与对照组相比，添加纤维素酶显著提高了粗蛋白（CP）、水溶性碳水化合物（WSC）、乳酸菌（LAB）数量，同时显著降低了中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）含量（P<0.05）。添加漆酶显著降低了酸性洗涤木质素（ADL）含量（P<0.05）。纤维素酶和漆酶联合应用显著提高了CP、WSC含量及LAB数量，同时显著降低了pH值、NDF、ADF和ADL含量（P<0.05），从而改善了发酵品质。此外，纤维素酶和漆酶的应用增加了厚壁菌门和LAB属的丰度，降低了样品的微生物多样性水平。
纤维素酶和漆酶联合应用进一步改善了玉米秸秆与啤酒糟混合青贮的发酵品质和微生物群落。
