Zhao Guoqiang, Wu Hao, Li Li, He Jiajun, Hu Zhichao, Yang Xinjian, Xie Xiangxue
Ruminant Product Research and Development Department, Guangdong VTR Bio-Tech, Zhuhai 519060, China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
J Anim Sci Technol. 2021 Nov;63(6):1301-1313. doi: 10.5187/jast.2021.e107. Epub 2021 Nov 30.
This study investigated the effects of applying cellulase and starch on the fermentation characteristics and microbial communities of Napier grass silage after ensiling for 30 d. Three groups were studied: No additives (control); added cellulase (Group 1); and added cellulase and starch (Group 2). The results showed that the addition of cellulase and starch decreased the crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and pH significantly ( < 0.05) and increased water-soluble carbohydrate (WSC) content ( < 0.05). The addition of additives in two treated groups exerted a positive effect on the lactic acid (LA) content, lactic acid bacteria (LAB) population, and lactic acid / acetic acid (LA/AA) ratio, even the changes were not significant ( > 0.05). Calculation of Flieg's scores indicated that cellulase application increased silage quality to some extent, while the application of cellulase and starch together significantly improved fermentation ( < 0.05). Compared with the control, both additive groups showed increased microbial diversity after ensiling with an abundance of favorable bacteria including Firmicutes and , and the bacteria including Proteobacteria, Bacteroidetes, increased as well. For alpha diversity analysis, the combined application of cellulase and starch in Group 2 gave significant increases in all indices ( < 0.05). The study demonstrated that the application of cellulase and starch can increase the quality of Napier grass preserved as silage.
本研究调查了添加纤维素酶和淀粉对象草青贮30天后发酵特性和微生物群落的影响。研究了三组：不添加添加剂（对照组）；添加纤维素酶（第1组）；添加纤维素酶和淀粉（第2组）。结果表明，添加纤维素酶和淀粉显著降低了粗蛋白（CP）、中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）含量和pH值（P<0.05），并提高了水溶性碳水化合物（WSC）含量（P<0.05）。两个处理组中添加剂的添加对乳酸（LA）含量、乳酸菌（LAB）数量和乳酸/乙酸（LA/AA）比值产生了积极影响，尽管变化不显著（P>0.05）。弗利格评分计算表明，添加纤维素酶在一定程度上提高了青贮品质，而同时添加纤维素酶和淀粉显著改善了发酵效果（P<0.05）。与对照组相比，两个添加剂组在青贮后微生物多样性均增加，包括厚壁菌门和……等有益菌数量增加，变形菌门、拟杆菌门等细菌数量也增加。对于α多样性分析，第2组中纤维素酶和淀粉的联合应用使所有指标均显著增加（P<0.05）。该研究表明，添加纤维素酶和淀粉可以提高象草青贮的品质。 （注：原文中“Firmicutes and ”以及“ the bacteria including Proteobacteria, Bacteroidetes, ”部分表述不完整，翻译时尽量保留了原文结构）
