Boghdady Wesam A, Khairy Marwa A, Ali Ali G, El Shahawy Alia A, Abdelaziz Eman A, El Shahawy Aya A, Kamel Fatma Z
Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Department of Ophthalmology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Front Allergy. 2024 Sep 25;5:1437600. doi: 10.3389/falgy.2024.1437600. eCollection 2024.
The genetic variants that alter human Forkhead Box P3 () function may have a part in the establishment of allergic conjunctivitis. Our study aimed to evaluate the polymorphism, serum interleukin13 (IL13) and total immunoglobulin E (IgE) levels in allergic conjunctivitis and assess their role as biomarkers for allergic conjunctivitis risk and severity.
This study included 52 cases and 52 controls. Blood samples were taken from allergic conjunctivitis patients and controls for total IgE, IL13 measurement and detection of (rs3761548) gene polymorphism.
There was a statistically significant difference between the allergic conjunctivitis group and healthy control group regarding (rs3761548) polymorphism with those have AA genotype are 12 times at risk for allergic conjunctivitis and A allele increases the risk of allergic conjunctivitis by about 4 times. There was statistically significant difference between mild/moderate and severe allergic conjunctivitis regarding (rs3761548) polymorphism with those have AA genotype are 53 times at risk for severe allergic conjunctivitis and A allele increases the risk of severe allergic conjunctivitis by about 6 times. Also, there was a significantly higher value of total IgE IU/ml, IL13 Pg/ml value in severe allergic conjunctivitis compared to moderate/mild allergic conjunctivitis. The best cutoff values of total IgE and serum IL13 for detecting the severity of allergic conjunctivitis were ≥320 IU/ml and ≥40 Pg/ml and the area under the curve were 0.89 and 0.95 respectively.
The research significantly contributes to find correlation of polymorphism, total IgE and IL13 with risk and severity of allergic conjunctivitis which are limited in the literature on the perceived value relevance of polymorphism in allergic conjunctivitis risk and severity.
改变人类叉头框蛋白P3（FOXP3）功能的基因变异可能在过敏性结膜炎的发病过程中起作用。我们的研究旨在评估FOXP3基因多态性、血清白细胞介素13（IL13）和总免疫球蛋白E（IgE）水平在过敏性结膜炎中的情况，并评估它们作为过敏性结膜炎风险和严重程度生物标志物的作用。
本研究纳入52例病例和52例对照。采集过敏性结膜炎患者和对照的血样，检测总IgE、IL13水平并检测FOXP3（rs3761548）基因多态性。
过敏性结膜炎组与健康对照组在FOXP3（rs3761548）多态性方面存在统计学显著差异，AA基因型个体患过敏性结膜炎的风险是对照组的12倍，A等位基因使过敏性结膜炎风险增加约4倍。轻度/中度与重度过敏性结膜炎在FOXP3（rs3761548）多态性方面存在统计学显著差异，AA基因型个体患重度过敏性结膜炎的风险是对照组的53倍，A等位基因使重度过敏性结膜炎风险增加约6倍。此外，重度过敏性结膜炎患者的总IgE（IU/ml）和IL13（Pg/ml）值显著高于中度/轻度过敏性结膜炎患者。检测过敏性结膜炎严重程度的总IgE和血清IL13的最佳截断值分别为≥320 IU/ml和≥40 Pg/ml，曲线下面积分别为0.89和0.95。
本研究显著有助于发现FOXP3基因多态性、总IgE和IL13与过敏性结膜炎风险和严重程度之间的相关性，而关于FOXP3基因多态性在过敏性结膜炎风险和严重程度方面的潜在价值相关性，现有文献报道有限。
