Hafez Raghda Abdellatif, Hassan Manar E, Haggag Maha G, Atef Nora, Abdallah Alshimaa L, Gerges Marian A
Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Microbiology, Immunology and Parasitology Department, Research Institute of Ophthalmology, Giza, Egypt.
J Asthma Allergy. 2022 Jul 26;15:971-982. doi: 10.2147/JAA.S373098. eCollection 2022.
Single nucleotide polymorphisms (SNPs) in the interleukin 13 (IL13) gene are associated with vulnerability to allergic diseases, such as asthma and allergic conjunctivitis (AC). Periostin, as an IL13-induced protein, has emerged as a novel biomarker in several allergic diseases. Data among Egyptian patients are still scarce.
To find out the association of IL13 rs20541 gene polymorphism and serum levels of periostin with asthma and AC among Egyptian patients.
Eighty-one Egyptian allergic patients with asthma, AC, and both asthma and AC (27 each), were enrolled in this case-control study. Twenty-seven age and gender-matched healthy volunteers served as controls. All participants were tested for IL13 rs20541 SNP by real-time polymerase chain reaction, TaqMan method. Serum levels of periostin and IL13 were assessed by ELISA.
Compared to healthy subjects, asthmatic patients had a higher frequency of the homozygous adenine/adenine (AA) genotype at IL13 rs20541 SNP (14.8% vs 3.7%) and a lower frequency of the guanosine/guanosine (GG) genotype (51.9% vs 55.6%), while AC patients had higher GG genotype (70.4% vs 55.6%) with no AA genotype detected, yet no significant difference was noticed (p = 7.053). A significantly higher serum periostin in asthmatic patients compared to controls was found (p = 0.005). Higher levels of serum periostin, although nonsignificant, were recorded in AC patients compared to controls (22.88 ± 10.01ng/mL and 17.51 ± 3.17ng/mL, respectively). Periostin was significantly higher in patients with IL13 AA and GA genotypes compared to those with GG genotype (p = 0.016). A significant positive correlation between serum periostin and serum IL13 among allergic patients was recorded (r = 0.352, p < 0.001).
Among Egyptian patients, serum level of periostin is significantly associated with asthma and positively correlates with IL13 level supporting its utility as a diagnostic biomarker. IL13 rs20541 gene polymorphism does not seem to play an obvious role in asthma and AC, which requires further evaluation.
白细胞介素13（IL13）基因中的单核苷酸多态性（SNP）与哮喘和过敏性结膜炎（AC）等过敏性疾病的易感性相关。骨膜蛋白作为一种IL13诱导的蛋白，已成为几种过敏性疾病中的一种新型生物标志物。埃及患者的数据仍然很少。
探讨埃及患者中IL13 rs20541基因多态性和骨膜蛋白血清水平与哮喘和AC的关系。
81例患有哮喘、AC以及同时患有哮喘和AC的埃及过敏性患者（各27例）被纳入本病例对照研究。27名年龄和性别匹配的健康志愿者作为对照。通过实时聚合酶链反应（TaqMan法）对所有参与者进行IL13 rs20541 SNP检测。采用酶联免疫吸附测定法（ELISA）评估骨膜蛋白和IL13的血清水平。
与健康受试者相比，哮喘患者在IL13 rs20541 SNP处纯合子腺嘌呤/腺嘌呤（AA）基因型的频率更高（14.8%对3.7%），鸟苷/鸟苷（GG）基因型的频率更低（51.9%对55.6%），而AC患者GG基因型频率更高（70.4%对55.6%），未检测到AA基因型，但差异无统计学意义（p = 7.053）。发现哮喘患者血清骨膜蛋白水平显著高于对照组（p = 0.005）。与对照组相比，AC患者血清骨膜蛋白水平虽无统计学意义但有所升高（分别为22.88±10.01ng/mL和17.51±3.17ng/mL）。与GG基因型患者相比，IL13 AA和GA基因型患者的骨膜蛋白水平显著更高（p = 0.016）。记录到过敏性患者血清骨膜蛋白与血清IL13之间存在显著正相关（r = 0.352，p < 0.001）。
在埃及患者中，骨膜蛋白血清水平与哮喘显著相关，且与IL13水平呈正相关，支持其作为诊断生物标志物的效用。IL13 rs20541基因多态性在哮喘和AC中似乎未发挥明显作用，这需要进一步评估。
