Cetin Nihal, Menevse Esma, Ceylan Cengizhan, Celik Zeliha Esin, Akdam Neriman, Rama Seyma Tetik, Buyukyildirim Tugsen, Pasayeva Leyla, Tugay Osman, Gumus Meltem
Department of Pharmacology, Faculty of Medicine, Selcuk University, 42131, Konya, Turkey.
Department of Biochemistry, Faculty of Medicine, Selcuk University, 42131, Konya, Turkey.
Iran J Basic Med Sci. 2024;27(11):1464-1474. doi: 10.22038/ijbms.2024.78382.16941.
Some species of L. are popularly used to treat gastric ulcers. However, the possible healing mechanisms of the anti-ulcer activity of , which has proven antioxidant, anti-inflammatory, and wound-healing properties, are unclear.
Ethanol extracts of fruits were administered orally at 100 mg/kg and 200 mg/kg to Wistar albino rats, with an indomethacin-induced gastric ulcer model. The ulcerous areas on the stomach surface were examined macroscopically. Tissues were examined histopathologically and biochemically. LC-HRMS revealed the phytochemical content.
TNF-α, IL-6, IL-1β, IL-8, and NF-kB levels were higher in the gastric ulcer group than in the extract groups. The VEGF values did not differ in each group. A significant difference was found between the lansoprazole group and the high-dose group regarding PGE2 levels. A histopathologically significant difference was observed between the healthy group and the indomethacin-applied groups in terms of neutrophilic infiltration of the gastric mucosa. Ascorbic acid (1547.521 µg/g), homoprotocatechuic acid (1268.217 µg/g), and genistein (1014.462 µg/g) were found as the main compounds in the extract by LC-HRMS.
Our results demonstrated that protected the gastric mucosa from inflammation and also modulated the PGE2 pathway. When considered in terms of TNF-α, IL-1β, IL-8, IL-6, PGE2, and NF-kB values, it can be concluded that it has a similar or even more positive effect than the reference substance. showed its effects in a dose-dependent manner.
某些罗勒属植物常用于治疗胃溃疡。然而，罗勒属植物具有抗氧化、抗炎和伤口愈合特性，其抗溃疡活性的可能愈合机制尚不清楚。
将罗勒属植物果实的乙醇提取物以100mg/kg和200mg/kg的剂量口服给予Wistar白化大鼠，建立吲哚美辛诱导的胃溃疡模型。肉眼检查胃表面的溃疡区域。对组织进行组织病理学和生化检查。液相色谱-高分辨质谱分析揭示了植物化学成分。
胃溃疡组的肿瘤坏死因子-α、白细胞介素-6、白细胞介素-1β、白细胞介素-8和核因子-κB水平高于提取物组。各组间血管内皮生长因子值无差异。兰索拉唑组和高剂量罗勒属植物提取物组之间的前列腺素E2水平存在显著差异。在胃黏膜嗜中性粒细胞浸润方面，健康组和应用吲哚美辛的组之间在组织病理学上存在显著差异。通过液相色谱-高分辨质谱分析发现，抗坏血酸（1547.521μg/g）、高香草酸（1268.217μg/g）和染料木黄酮（1014.462μg/g）是罗勒属植物提取物中的主要化合物。
我们的结果表明，罗勒属植物保护胃黏膜免受炎症侵害，并调节前列腺素E2途径。从肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-8、白细胞介素-6、前列腺素E2和核因子-κB值来看，可以得出结论，它比参考物质具有相似甚至更积极的作用。罗勒属植物以剂量依赖的方式显示出其作用。
