质粒介导的抗生素耐药性从链球菌向丙酮丁醇梭菌的接合转移。

Conjugal transfer of plasmid-mediated antibiotic resistance from streptococci to Clostridium acetobutylicum.

作者信息

Reysset G, Sebald M

出版信息

Ann Inst Pasteur Microbiol (1985). 1985 Nov-Dec;136B(3):275-82. doi: 10.1016/s0769-2609(85)80073-9.

Abstract

Three broad host-range MLS plasmids, pAM beta 1 (Em), pIP501 (Em, Cm) and pJH4 (Em, Sm, Km), were transferred by a conjugation-like process from Streptococcus faecalis to Clostridium acetobutylicum. The plasmids were stably maintained and antibiotic resistances were fully expressed in C. acetobutylicum, except for chloramphenicol resistance, which was poorly expressed, if at all. The C. acetobutylicum strains harbouring every plasmid could be used as donors in intraspecific matings with frequencies of approximately 1 X 10(-5) to 1 X 10(-6).

摘要

三种广泛宿主范围的大环内酯-林可酰胺-链阳菌素（MLS）质粒，即pAMβ1（红霉素抗性）、pIP501（红霉素、氯霉素抗性）和pJH4（红霉素、链霉素、卡那霉素抗性），通过一种类似接合的过程从粪肠球菌转移到丙酮丁醇梭菌。这些质粒在丙酮丁醇梭菌中稳定维持，除氯霉素抗性表达不佳（即便有表达）外，抗生素抗性在该菌中完全表达。携带每种质粒的丙酮丁醇梭菌菌株可用作种内交配的供体，交配频率约为1×10⁻⁵至1×10⁻⁶。