用于评估抗病毒干预措施的荧光和生物发光牛 H5N1 流感病毒。
Fluorescent and bioluminescent bovine H5N1 influenza viruses for evaluation of antiviral interventions.
机构信息
Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA.
Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA.
出版信息
J Virol. 2024 Nov 19;98(11):e0138524. doi: 10.1128/jvi.01385-24. Epub 2024 Oct 10.
Abstract
In early 2024, a clade 2.3.4.4b high pathogenic H5N1 avian influenza virus was detected in dairy cows and humans in the United States. Since then, it has spread to herds in at least 13 states and caused symptomatic disease in at least fifteen people. To facilitate rapid testing of existing and novel countermeasures, here, we report the development of an H5N1 viral reverse genetic system, its use to produce fluorescent and bioluminescent variant strains, and their utility in high-throughput evaluation of antiviral interventions.
摘要
2024 年初，在美国的奶牛和人类中检测到了一个 2.3.4.4b 分支的高致病性 H5N1 禽流感病毒。此后，它已传播到至少 13 个州的牛群中，并导致至少 15 人出现症状性疾病。为了便于快速测试现有和新型对策，在此，我们报告了 H5N1 病毒反向遗传系统的开发，以及利用该系统生产荧光和生物发光变异株及其在高通量评估抗病毒干预措施中的应用。
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2
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Emerg Infect Dis. 2024 Jul;30(7):1335-1343. doi: 10.3201/eid3007.240508. Epub 2024 Apr 29.
3
Segmented, Negative-Sense RNA Viruses of Humans: Genetic Systems and Experimental Uses of Reporter Strains.人类分段负义 RNA 病毒：报告株的遗传系统和实验用途。
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4
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6
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7
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9
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