Enterovibrio qingdaonensis sp. nov. and Enterovibrio gelatinilyticus sp. nov., two marine bacteria isolated from surface seawater of Qingdao offshore.

Two Gram-stain-negative, facultatively anaerobic, motile, and rod-shaped marine bacteria, designated strains ZSDZ35 and ZSDZ42, were isolated from surface seawater of Qingdao offshore. Phylogenetic analysis of the 16S rRNA genes and whole genome data placed ZSDZ35 and ZSDZ42 within the genus Enterovibrio. Strain ZSDZ35 was most closely related to Enterovibrio nigricans DSM 22720 with 97.55% sequence similarity, whereas ZSDZ42 was most closely related to Enterovibrio calviensis DSM 14347 with 98.97% sequence similarity. Strain ZSDZ35 grew with 0-8% (w/v) NaCl (optimum 4%), at 16-37 °C (optimum 28 °C) and pH 6.0-9.0 (optimum pH 8.0); whereas strain ZSDZ42 grew with 1-7% (w/v) NaCl (5%), at 4-28 °C (8 °C) and pH 6.0-9.0 (pH 7.0). Both strains shared the same major fatty acid components of summed feature 3 (Cω7c or/and Cω6c), summed feature 8 (Cω7c and Cω6c) and C, with different proportions. The DNA G + C contents of strains ZSDZ35 and ZSDZ42 were 47.2% and 46.7%, respectively. Based on the results of polyphasic analyses, ZSDZ35 and ZSDZ42 are considered to represent novel species, for which the names Enterovibrio qingdaonensis sp. nov. (type strain, ZSDZ35 = MCCC 1K06293 = KCTC 82887) and Enterovibrio gelatinilyticus sp. nov. (type strain, ZSDZ42 = MCCC 1K06294 = KCTC 82886) are proposed, respectively.