Zhang Pingping, Wang Xiaolei, Liu Ronghua, Zhu Xiaoyu, Zhao Wenbin, Zhang Xiao-Hua
College of Marine Life Sciences and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005674.
Two Gram-stain-negative, oxidase-positive, facultative anaerobic and rod-shaped motile bacteria, designated strains ZSDZ34 and ZSDE26, were isolated from offshore surface seawater collected near Qingdao. Phylogenetic analysis based on 16S rRNA gene sequences placed ZSDE26 and ZSDZ34 within the genus , family , class . Strain ZSDE26 was most closely related to VB 8.9 with 97.3 % sequence similarity, whereas ZSDZ34 was most closely related to subsp. DSM 109723 with 97.8 % sequence similarity. Strain ZSDE26 grew with 1-5 % (w/v) NaCl (optimum, 4 %), at 16-28 °C (optimum, 28 °C) and at pH 6.0-9.0 (optimum, pH 7.0). Growth of strain ZSDZ34 occurred with 1-6 % (w/v) NaCl (optimum, 3 %), at 16-37 °C (optimum, 28 °C) and at pH 6.0-9.0 (optimum, pH 7.0). Both strains shared the same major fatty acid components (more than 10 % of total fatty acids) of summed feature 3 (C 7 and/or C 6), summed feature 8 (C 7 and/or C 6) and C. Additionally, strain ZSDZ34 contained a higher proportion of iso-C. The DNA G+C contents of strains ZSDE26 and ZSDZ34 were 42.8 and 44.5 mol%, respectively. On the basis of the results of polyphasic analysis, ZSDE26 and ZSDZ34 are considered to represent novel species within the genus , for which the names sp. nov. (type strain, ZSDE26=KCTC 82890=MCCC 1K06290) and sp. nov. (type strain, ZSDZ34=KCTC 82888=MCCC 1K06292) are proposed, respectively.
从青岛附近采集的近海表层海水中分离出两株革兰氏阴性、氧化酶阳性、兼性厌氧、杆状且具运动性的细菌，分别命名为ZS DZ34和ZS D E26菌株。基于16S rRNA基因序列的系统发育分析将ZS D E26和ZS DZ34置于 属、 科、 纲内。ZS D E26菌株与VB 8.9的亲缘关系最为密切，序列相似性为97.3%，而ZS DZ34与 亚种DSM 109723的亲缘关系最为密切，序列相似性为97.8%。ZS D E26菌株在含1 - 5%（w/v）NaCl（最适浓度为4%）、温度为16 - 28°C（最适温度为28°C）、pH值为6.0 - 9.0（最适pH值为7.0）的条件下生长。ZS DZ34菌株在含1 - 6%（w/v）NaCl（最适浓度为3%）、温度为16 - 37°C（最适温度为28°C）、pH值为6.0 - 9.0（最适pH值为7.0）的条件下生长。两株菌株具有相同的主要脂肪酸成分（占总脂肪酸的10%以上），即特征性总和3（C 7和/或C 6）、特征性总和8（C 7和/或C 6）和C。此外，ZS DZ34菌株含有较高比例的异-C。ZS D E26和ZS DZ34菌株的DNA G+C含量分别为42.8和44.5 mol%。基于多相分析结果，ZS D E26和ZS DZ34被认为代表 属内的新物种，分别提议将其命名为 新种（模式菌株，ZS D E26 = KCTC 82890 = MCCC 1K06290）和 新种（模式菌株，ZS DZ34 = KCTC 82888 = MCCC 1K06292）。
