sp. nov. and sp. nov., two marine bacteria isolated from surface seawater of Qingdao.

Two Gram-stain-negative, oxidase-positive, facultative anaerobic and rod-shaped motile bacteria, designated strains ZSDZ34 and ZSDE26, were isolated from offshore surface seawater collected near Qingdao. Phylogenetic analysis based on 16S rRNA gene sequences placed ZSDE26 and ZSDZ34 within the genus , family , class . Strain ZSDE26 was most closely related to VB 8.9 with 97.3 % sequence similarity, whereas ZSDZ34 was most closely related to subsp. DSM 109723 with 97.8 % sequence similarity. Strain ZSDE26 grew with 1-5 % (w/v) NaCl (optimum, 4 %), at 16-28 °C (optimum, 28 °C) and at pH 6.0-9.0 (optimum, pH 7.0). Growth of strain ZSDZ34 occurred with 1-6 % (w/v) NaCl (optimum, 3 %), at 16-37 °C (optimum, 28 °C) and at pH 6.0-9.0 (optimum, pH 7.0). Both strains shared the same major fatty acid components (more than 10 % of total fatty acids) of summed feature 3 (C 7 and/or C 6), summed feature 8 (C 7 and/or C 6) and C. Additionally, strain ZSDZ34 contained a higher proportion of iso-C. The DNA G+C contents of strains ZSDE26 and ZSDZ34 were 42.8 and 44.5 mol%, respectively. On the basis of the results of polyphasic analysis, ZSDE26 and ZSDZ34 are considered to represent novel species within the genus , for which the names sp. nov. (type strain, ZSDE26=KCTC 82890=MCCC 1K06290) and sp. nov. (type strain, ZSDZ34=KCTC 82888=MCCC 1K06292) are proposed, respectively.