Fu Juan, Meng Kun, Yuan Qingmin
Department of Stomatology, Henan Vocational College of Nursing, Anyang, 455000, Henan Province, China.
Department of Prosthodontic, Anyang Stomatological Hospital, Anyang, 455000, Henan Province, China.
Cell Biochem Biophys. 2025 Jun;83(2):1713-1723. doi: 10.1007/s12013-024-01580-7. Epub 2024 Oct 10.
It was to clarify the effects of silver nanoparticles (AgNPs) on biological functions of human periodontal ligament fibroblasts (hPDLFs).
AgNPs were synthesized using a tannic acid reduction method and characterized accordingly. Fifteen Sprague-Dawley rats were randomly assigned to Normal group, Group A (orthodontic tooth movement after alveolar bone defect repair with a blood clot), and Group B (orthodontic tooth movement after alveolar bone defect repair with AgNPs), with five rats in each group. Morphological changes in periodontal tissues were visualized. hPDLFs were treated with 0 μM (Ctrl), 25 μM (L-AgNPs), 50 μM (M-AgNPs), and 100 μM (H-AgNPs) AgNPs to assess cell proliferation via the MTT assay, calcification via alizarin red staining, and osteogenic differentiation and genes/proteins' expression associated with the I3K/Akt signaling pathway through quantitative polymerase chain reaction and Western blot.
AgNP diameter was approximately 20 nm. Relative to the normal group, both Group A and Group B exhibited increased widths of the periodontal ligament (PDL) while displaying a decrease in cell counts within the PDL (P < 0.05). Furthermore, the L-AgNPs, M-AgNPs, and H-AgNPs groups exhibited a notable elevation in the number of calcified nodules in hPDLFs, along with elevated alkaline phosphatase, Runx2, osteocalcin, osterix, type I collagen, phosphorylated phosphoinositide 3-kinase, and phosphorylated protein kinase B versus Ctrl (P < 0.05).
AgNPs are beneficial in enhancing the biological functions of the PDL, promoting the repair and regeneration of periodontal tissues, indicating their potential clinical value in orthodontic treatments.
本研究旨在阐明银纳米颗粒（AgNPs）对人牙周膜成纤维细胞（hPDLFs）生物学功能的影响。
采用单宁酸还原法合成AgNPs并进行相应表征。将15只Sprague-Dawley大鼠随机分为正常组、A组（牙槽骨缺损用血凝块修复后正畸牙齿移动）和B组（牙槽骨缺损用AgNPs修复后正畸牙齿移动），每组5只大鼠。观察牙周组织的形态学变化。用0 μM（对照）、25 μM（低剂量AgNPs，L-AgNPs）、50 μM（中剂量AgNPs，M-AgNPs）和100 μM（高剂量AgNPs，H-AgNPs）的AgNPs处理hPDLFs，通过MTT法评估细胞增殖，通过茜素红染色评估钙化，通过定量聚合酶链反应和蛋白质免疫印迹法评估与I3K/Akt信号通路相关的成骨分化及基因/蛋白质表达。
AgNP直径约为20 nm。与正常组相比，A组和B组的牙周膜（PDL）宽度均增加，而PDL内细胞计数减少（P < 0.05）。此外，L-AgNPs、M-AgNPs和H-AgNPs组的hPDLFs中钙化结节数量显著增加，碱性磷酸酶、Runx2、骨钙素、osterix、I型胶原蛋白、磷酸化磷脂酰肌醇3-激酶和磷酸化蛋白激酶B水平相对于对照组也升高（P < 0.05）。
AgNPs有利于增强PDL的生物学功能，促进牙周组织的修复和再生，表明其在正畸治疗中的潜在临床价值。
