Mackiewicz A, Wiktorowicz K, Mackiewicz S
Arch Immunol Ther Exp (Warsz). 1985;33(5):693-701.
Three widely used immunoassays for C-reactive protein (CRP) determination (RID. RIEP and LN--Behring) were compared. According to the CRP calcium dependent structural changes or ligand binding properties two buffer systems, Ca2+ rich and Ca2+ depleted, were used in all methods. In RID and RIEP mean values of studied samples were higher when Ca2+ depleted buffer was used (r = 0.94 and r = 0.88, respectively). However, in LN mean values of studied samples were higher when Ca2+ rich buffer was employed (r = 0.923). Comparison of RID and RIEP showed better correlation when Ca2+ depleted buffer was used with r = 0.913 and regression equation y = 0.92 chi + 2.76. Comparison of RID with LN gave similar results as in the case of RIEP and LN. In both cases mean values of studied samples were much higher in LN. Best correlation between RID and LN was obtained when RID was run in Ca2+ depleted buffer and LN performed in Ca2+ rich buffer (r = 0.845, y = 1.25 chi + 18.4). When RIEP and LN were compared highest correlation was obtained when Ca2+ rich buffer in both methods had been used (r = 0.84, y = 1.45 chi + 33.7). However, comparison of both methods run in Ca2+ depleted buffer yielded better regression equation: y = 1.05 chi + 26.1 with r = 0.795.
对三种广泛用于测定C反应蛋白（CRP）的免疫测定法（散射比浊法、火箭免疫电泳法和朗道 - 贝林法）进行了比较。根据CRP依赖钙的结构变化或配体结合特性，在所有方法中使用了两种缓冲系统，即富含Ca2+和缺乏Ca2+的缓冲系统。在散射比浊法和火箭免疫电泳法中，当使用缺乏Ca2+的缓冲液时，研究样本的平均值较高（相关系数分别为r = 0.94和r = 0.88）。然而，在朗道 - 贝林法中，当使用富含Ca2+的缓冲液时，研究样本的平均值较高（r = 0.923）。当使用缺乏Ca2+的缓冲液时，散射比浊法和火箭免疫电泳法的相关性更好，相关系数r = 0.913，回归方程为y = 0.92x + 2.76。散射比浊法与朗道 - 贝林法的比较结果与火箭免疫电泳法和朗道 - 贝林法的情况相似。在这两种情况下，朗道 - 贝林法中研究样本的平均值要高得多。当散射比浊法在缺乏Ca2+的缓冲液中进行，而朗道 - 贝林法在富含Ca2+的缓冲液中进行时，散射比浊法与朗道 - 贝林法之间的相关性最佳（r = 0.845，y = 1.25x + 18.4）。当比较火箭免疫电泳法和朗道 - 贝林法时，当两种方法都使用富含Ca2+的缓冲液时，相关性最高（r = 0.84，y = 1.45x + 33.7）。然而，对在缺乏Ca2+的缓冲液中进行的两种方法进行比较时，得到了更好的回归方程：y = 1.05x + 26.1，相关系数r = 0.795。
