Knecht H, Streuli R A
Acta Haematol. 1985;74(4):208-12. doi: 10.1159/000206220.
Megakaryocytopoiesis was investigated with a polyclonal (26 cases) and a monoclonal (20 cases) antibody to factor-VIII-related antigen (factor VIII RAg) with the indirect peroxidase-antiperoxidase (PAP) method on air-dried bone marrow aspirates (BM). Numerous megakaryocyte precursors were identified in 5 patients with essential thrombocythemia (ET) and the 2 patients with acute myelogeneous leukaemia (AML) after recovery from therapeutic aplasia. Small megakaryocyte precursors were rare in controls (C), patients with reactive thrombocytosis (RT) and immune thrombocytopenia (IT). In 2 patients with severe alcoholism the number of mature megakaryocytes increased after 5 and 7 days of abstinence, respectively.
采用间接过氧化物酶 - 抗过氧化物酶（PAP）法，使用针对因子 VIII 相关抗原（因子 VIII RAg）的多克隆抗体（26 例）和单克隆抗体（20 例），对空气干燥的骨髓穿刺液（BM）进行巨核细胞生成的研究。在 5 例原发性血小板增多症（ET）患者和 2 例治疗性再生障碍后恢复的急性髓性白血病（AML）患者中，发现了大量巨核细胞前体。在对照组（C）、反应性血小板增多症（RT）患者和免疫性血小板减少症（IT）患者中，小巨核细胞前体很少见。在 2 例严重酒精中毒患者中，分别在戒酒 5 天和 7 天后，成熟巨核细胞数量增加。
