van der Kwast T H, Stel H V, Cristen E, Bertina R M, Veerman E C
Blood. 1986 Jan;67(1):222-7.
Various organs, including liver, spleen, heart, lung, kidney, intestines, lymph nodes, pancreas, bone marrow, and thymus, were investigated for the presence of factor VIII-procoagulant antigen (VIIICAg) and factor VIII-related antigen (VIIIRAg), using a panel of monoclonal antibodies directed to factor VIII-von Willebrand factor in combination with a sensitive immunoperoxidase staining technique. In addition to hepatic sinusoidal endothelial cells, the presence of VIIICAg was demonstrated in mononuclear cells sporadically present in lymph nodes, in the alveolar septa of lung, and in the red pulp of spleen. The identity of these mononuclear cells could not be unequivocally determined. Based on morphological criteria, however, it is tentatively concluded that these cells are nonlymphoid and belong to the mononuclear phagocyte system. The presence of VIII-RAg was confined to vascular endothelial cells, hepatic sinusoidal endothelial cells, cells lining the venous sinuses of the red pulp of the spleen, cells lining renal glomeruli and lung capillaries, platelets, and megakaryocytes.
利用一组针对因子VIII-血管性血友病因子的单克隆抗体，并结合灵敏的免疫过氧化物酶染色技术，对包括肝脏、脾脏、心脏、肺、肾脏、肠道、淋巴结、胰腺、骨髓和胸腺在内的各种器官进行了检测，以确定是否存在因子VIII促凝抗原（VIIICAg）和因子VIII相关抗原（VIIIRAg）。除肝窦内皮细胞外，在淋巴结中散在的单核细胞、肺的肺泡隔以及脾脏的红髓中也证实存在VIIICAg。这些单核细胞的身份无法明确确定。然而，根据形态学标准，初步推断这些细胞是非淋巴细胞，属于单核吞噬细胞系统。VIII-RAg的存在局限于血管内皮细胞、肝窦内皮细胞、脾脏红髓静脉窦内衬的细胞、肾小球和肺毛细血管内衬的细胞、血小板和巨核细胞。
