Dai Yun, Zhang Shifan, Guan Jiantao, Wang Shaoxing, Zhang Hui, Li Guoliang, Sun Rifei, Li Fei, Zhang Shujiang
Hortic Res. 2024 Jul 30;11(10):uhae214. doi: 10.1093/hr/uhae214. eCollection 2024 Oct.
In Chinese cabbage development the interplay between shoot apex activity and vernalization is pivotal for flowering timing. The intricate relationship between various cell types in the shoot apex meristem and their roles in regulating flowering gene expression in Chinese cabbage is not yet fully understood. A thorough analysis of single-cell types in the Chinese cabbage shoot apex and their influence on flowering genes and vernalization is essential for deeper insight. Our study first established a single-cell transcriptomic atlas of Chinese cabbage after 25 days of non-vernalization. Analyzing 19 602 single cells, we differentiated them into 15 distinct cell clusters using established marker genes. We found that key genes in shoot apex development and flowering were primarily present in shoot meristematic cells (SMCs), companion cells (CCs), and mesophyll cells (MCs). (), a gene suppressing flowering, was observed in CCs, mirroring patterns found in . By mapping developmental trajectories of SMCs, CCs, and MCs, we elucidated the evolutionary pathways of crucial genes in shoot apex development and flowering. The creation of a single-cell transcriptional atlas of the Chinese cabbage shoot apex under vernalization revealed distinct alterations in the expression of known flowering genes, such as (), (), (), , and (), which varied by cell type. Our study underscores the transformative impact of single-cell RNA sequencing (scRNA-seq) for unraveling the complex differentiation and vernalization processes in the Chinese cabbage shoot apex. These insights are pivotal for enhancing breeding strategies and cultivation management of this vital vegetable.
在大白菜发育过程中，茎尖活性与春化作用之间的相互作用对于开花时间至关重要。大白菜茎尖分生组织中各种细胞类型之间的复杂关系及其在调节开花基因表达中的作用尚未完全了解。深入分析大白菜茎尖中的单细胞类型及其对开花基因和春化作用的影响对于更深入的理解至关重要。我们的研究首先建立了未春化25天后大白菜的单细胞转录组图谱。通过分析19602个单细胞，我们使用已建立的标记基因将它们分化为15个不同的细胞簇。我们发现，茎尖发育和开花的关键基因主要存在于茎分生细胞（SMC）、伴胞（CC）和叶肉细胞（MC）中。在CC中观察到一个抑制开花的基因（），这与在（）中发现的模式相似。通过绘制SMC、CC和MC的发育轨迹，我们阐明了茎尖发育和开花中关键基因的进化途径。春化条件下大白菜茎尖单细胞转录图谱的建立揭示了已知开花基因（如（）、（）、（）、（）和（））表达的明显变化，这些变化因细胞类型而异。我们的研究强调了单细胞RNA测序（scRNA-seq）对于揭示大白菜茎尖复杂分化和春化过程的变革性影响。这些见解对于加强这种重要蔬菜的育种策略和栽培管理至关重要。
