National Key Laboratory of Crop Genetics & Germplasm Innovation and Utilization, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of China, Nanjing Agricultural University, Nanjing 210095, China.
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2024 Feb 5;25(3):1924. doi: 10.3390/ijms25031924.
Vernalization plays a crucial role in the flowering and yield of Chinese cabbage, a process intricately influenced by long non-coding RNAs (lncRNAs). Our research focused on , , and , which emerged as key players in this process. These lncRNAs exhibited an inverse expression pattern to the flowering repressor genes () and () during vernalization, suggesting a complex regulatory mechanism. Notably, their expression in the shoot apex and leaves was confirmed through in fluorescent in situ hybridization (FISH). Furthermore, when these lncRNAs were overexpressed in , a noticeable acceleration in flowering was observed, unveiling functional similarities to 's (). This resemblance suggests a potentially conserved regulatory mechanism across species. This study not only enhances our understanding of lncRNAs in flowering regulation, but also opens up new possibilities for their application in agricultural practices.
春化作用在大白菜的开花和产量中起着关键作用，这个过程受到长链非编码 RNA（lncRNA）的复杂影响。我们的研究集中在 、 、 ，它们在这个过程中表现为关键参与者。这些 lncRNA 在春化过程中表现出与开花抑制基因 （）和 （）相反的表达模式，表明存在复杂的调控机制。值得注意的是，通过荧光原位杂交（FISH）在茎尖和叶片中证实了它们的表达。此外，当这些 lncRNA 在 中过表达时，观察到开花明显加速，与 's （）的功能相似。这种相似性表明在物种间存在潜在的保守调控机制。这项研究不仅增强了我们对开花调控中 lncRNA 的理解，也为它们在农业实践中的应用开辟了新的可能性。
