Li Shujuan, Zou Quan, Jiang Yao, Wang Yi, Ding Xiangdong
State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Laboratory of Animal Genetics, Breeding and Reproduction, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Front Cell Dev Biol. 2024 Sep 26;12:1361485. doi: 10.3389/fcell.2024.1361485. eCollection 2024.
The hair follicle (HF) is a micro-organ capable of regeneration. A HF cycle consists of an anagen, catagen and telogen. Abnormalities in the HF cycle can lead to many hair disorders such as hair loss. The pig is a good biomedical model, but there are few data on their HF cycle. The aim of this study was to classify the pig HF cycle and determine the feasibility of the pig as an animal model for human HF cycle.
Skin samples from 10 different postnatal (P) days Yorkshire pigs was collected to determine the key time points of the first HF cycle in pig by H&E staining, immunofluorescence staining, q-PCR and western-blot.
By morphological observation and detection of markers at different stages, pig HF cycle was classified into three main periods - the first anagen until P45, catagen (P45-P85), telogen (P85-P100), and next anagen (>P100). In addition, we examined the expression of important genes , , , et al. related to the HF cycle at different stages of pig HF, indicating that pig and human share similarities in morphology and marker gene expression patterns of HF cycle.
Our findings will facilitate the study of HF cycle and offer researchers a suitable model for human hair research.
毛囊（HF）是一种能够再生的微器官。一个毛囊周期包括生长期、退行期和休止期。毛囊周期异常会导致许多毛发疾病，如脱发。猪是一种良好的生物医学模型，但关于其毛囊周期的数据较少。本研究的目的是对猪的毛囊周期进行分类，并确定猪作为人类毛囊周期动物模型的可行性。
收集10只不同出生后（P）天数的约克夏猪的皮肤样本，通过苏木精-伊红染色、免疫荧光染色、q-PCR和蛋白质免疫印迹法确定猪第一个毛囊周期的关键时间点。
通过形态学观察和不同阶段标志物的检测，猪的毛囊周期分为三个主要时期——第一个生长期直至P45，退行期（P45-P85），休止期（P85-P100），以及下一个生长期（>P100）。此外，我们检测了猪毛囊不同阶段与毛囊周期相关的重要基因、、、等的表达，表明猪和人类在毛囊周期的形态和标志物基因表达模式上具有相似性。
我们的研究结果将有助于毛囊周期的研究，并为研究人员提供一个适合人类毛发研究的模型。
