Alwi Aedrianee Reeza, Mahat Naji Arafat, Salleh Faezah Mohd, Ishar Seri Mirianti, Kamaluddin Mohammad Rahim, Rashid Mohd Radzniwan A, Rahayu Karmilla Syed Hassan Sharifah Nany, Iyavoo Sasitaran
Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia.
Department of Chemistry Malaysia Johor State, Jalan Abdul Samad, 80100 Johor Bahru, Johor, Malaysia.
Heliyon. 2024 Sep 18;10(19):e38054. doi: 10.1016/j.heliyon.2024.e38054. eCollection 2024 Oct 15.
The utilization of X-chromosome short tandem repeats (X-STRs) for human identification particularly in resolving complex kinship cases has been advocated. Since, forensic statistical parameters vary among different populations, and because the X-STRs population data representing the diverse population of Peninsular Malaysia remain unavailable, the specific attempt reported here for the Malays (n = 224) and Chinese (n = 201) populations appears forensically relevant to support the evidential value of the 12 X-STRs markers for human identification in Malaysia. Results derived from the Qiagen Investigator® Argus X-12 kit revealed that DXS10135 as the most polymorphic locus with high genetic diversity, polymorphic information content, heterozygosity as well as power of exclusion. Based on allele frequencies, the combined power of discrimination as well as the mean exclusion chance (MEC, MEC, MEC and MEC) values for the Malays and Chinese were individually ≥0.999995532964908. As for the combined power of discrimination as well as the mean exclusion chance (MEC, MEC, MEC and MEC) calculated based on haplotype frequencies, the values were ≥0.9999986410567 for the Malays and Chinese populations. In addition, results from the genetic distance, neighbor-joining phylogenetic tree and principal component analysis revealed close biogeographical distributions of the studied populations with other South East Asian populations. Hence, the utilization of the X-STRs data for identifying individuals among the Malays and Chinese populations in Peninsular Malaysia for forensic application appears as highly supported.
有人主张利用X染色体短串联重复序列（X-STRs）进行人类身份鉴定，特别是在解决复杂的亲属关系案件中。由于法医统计参数在不同人群中有所不同，而且代表马来西亚半岛不同人群的X-STRs群体数据仍然无法获得，因此本文报道的针对马来人（n = 224）和华人（n = 201）群体的具体尝试在法医方面似乎具有相关性，以支持12个X-STRs标记在马来西亚进行人类身份鉴定的证据价值。来自Qiagen Investigator® Argus X-12试剂盒的结果显示，DXS10135是多态性最高的位点，具有高遗传多样性、多态信息含量、杂合度以及排除能力。基于等位基因频率，马来人和华人的联合鉴别能力以及平均排除机会（MEC、MEC、MEC和MEC）值分别≥0.999995532964908。至于基于单倍型频率计算的联合鉴别能力以及平均排除机会（MEC、MEC、MEC和MEC），马来人和华人种群的值≥0.9999986410567。此外，遗传距离、邻接法系统发育树和主成分分析的结果显示，所研究的种群与其他东南亚种群在生物地理分布上相近。因此，利用X-STRs数据在马来西亚半岛的马来人和华人种群中进行个体识别以用于法医应用似乎得到了有力支持。
