Gui Biyu, Wang Qi, Wang Jianhai, Li Xue, Wu Qi, Chen Huaiyong
Department of Respiratory Medicine, Haihe Clinical School, Tianjin Medical University, Tianjin, 300350, China.
Department of Basic Medicine, Haihe Hospital, Tianjin University, Tianjin, 300350, China.
Heliyon. 2024 Sep 22;10(19):e38259. doi: 10.1016/j.heliyon.2024.e38259. eCollection 2024 Oct 15.
Studies of lung transcriptomics across species are essential for understanding the complex biology and disease mechanisms of this vital organ. Single-cell RNA sequencing (scRNA-seq) has emerged as a key tool for understanding cell dynamics across various species. However, comprehensive cross-species comparisons are limited. Therefore, the aims of this study was to investigate the transcriptomic similarities and differences in lung cells across four species-humans, monkeys, mice, and rats-in healthy and asthma conditions using scRNA-seq. The results revealed significant transcriptomic similarities between monkeys and humans and significant cross-species conservation of cell-specific marker genes, transcription factors (TFs), and biological pathways. Additionally, we explored sex differences, identifying distinct sex-specific expression patterns that may influence disease susceptibility. These insights refine our understanding of the mechanism underlying airway cell biology across species and have important implications for studying lung diseases, particularly the mechanisms of mucus clearance in asthma.
跨物种的肺转录组学研究对于理解这个重要器官的复杂生物学和疾病机制至关重要。单细胞RNA测序（scRNA-seq）已成为理解不同物种细胞动态的关键工具。然而，全面的跨物种比较仍然有限。因此，本研究的目的是使用scRNA-seq研究在健康和哮喘状态下，人类、猴子、小鼠和大鼠这四个物种的肺细胞转录组的异同。结果显示，猴子和人类之间存在显著的转录组相似性，并且细胞特异性标记基因、转录因子（TFs）和生物学途径存在显著的跨物种保守性。此外，我们还探索了性别差异，确定了可能影响疾病易感性的不同性别特异性表达模式。这些见解深化了我们对跨物种气道细胞生物学潜在机制的理解，并对研究肺部疾病，特别是哮喘中的黏液清除机制具有重要意义。
