Eder Lihi, Caucheteux Stephan M, Afiuni-Zadeh Somaieh, Croitoru David, Krizova Adriana, Limacher James J, Ritchlin Christopher, Jackson Hartland, Piguet Vincent
Department of Medicine, Women's College Hospital, Toronto, Canada; Department of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, Canada.
Department of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, Canada.
J Invest Dermatol. 2025 Jun;145(6):1361-1370. doi: 10.1016/j.jid.2024.08.039. Epub 2024 Oct 10.
Imaging mass cytometry is a technology that enables comprehensive analysis of cellular phenotypes at the tissue level. We performed a multiparameter characterization of structural and immune cell populations in psoriatic skin and synovial tissue samples aimed at characterizing immune cell differences in patients with psoriasis and psoriatic arthritis. A panel of 33 antibodies was used to stain selected immune and structural cell populations. Imaging mass cytometry data were segmented into single cells on the basis of combinations of antibody stains. Single cells were then clustered into cell categories on the basis of prespecified markers. The spatial relationships of different cell populations were assessed using neighborhood analysis. Among all cell types in the skin and synovium, lymphoid cells accounted for the most prevalent cell type. T cells and macrophages were the most prevalent immune cell type in the synovium, and B cells and NK cells were also identified. Neighborhood analysis showed high correlation between synovial T cells, B cells, macrophages, dendritic cells, and neutrophils, suggesting spatial organization. Innate and adaptive immune cells can be reliably identified using imaging mass cytometry in the skin and synovium. Interpatient heterogeneity exists in tissue cell populations. Imaging mass cytometry provides opportunities for exploring in depth the underlying immunological mechanisms driving psoriasis and psoriatic arthritis.
成像质谱流式细胞术是一种能够在组织水平对细胞表型进行全面分析的技术。我们对银屑病皮肤和滑膜组织样本中的结构细胞和免疫细胞群体进行了多参数表征，旨在明确银屑病和银屑病关节炎患者的免疫细胞差异。使用一组33种抗体对选定的免疫细胞和结构细胞群体进行染色。成像质谱流式细胞术数据基于抗体染色组合被分割为单个细胞。然后根据预先指定的标记将单个细胞聚类为细胞类别。使用邻域分析评估不同细胞群体的空间关系。在皮肤和滑膜的所有细胞类型中，淋巴细胞是最普遍的细胞类型。T细胞和巨噬细胞是滑膜中最普遍的免疫细胞类型，同时也鉴定出了B细胞和NK细胞。邻域分析显示滑膜T细胞、B细胞、巨噬细胞、树突状细胞和中性粒细胞之间具有高度相关性，提示存在空间组织。使用成像质谱流式细胞术能够在皮肤和滑膜中可靠地鉴定先天性和适应性免疫细胞。组织细胞群体存在患者间异质性。成像质谱流式细胞术为深入探索驱动银屑病和银屑病关节炎的潜在免疫机制提供了机会。
