Metabolism and Systems Science, University of Birmingham, Birmingham, UK.
Centre of Membrane Proteins and Receptors (COMPARE), Universities of Birmingham and Nottingham, Birmingham, UK.
Methods Mol Biol. 2025;2861:57-69. doi: 10.1007/978-1-0716-4164-4_5.
This protocol chapter outlines a guide for investigating ghrelin receptor activation of Gq signaling using Bioluminescence Resonance Energy Transfer (BRET). Focusing on the eBRET and NanoBRET™ variants, the chapter covers steps from cell culture to transfection, ligand stimulation, and BRET measurements, offering a robust protocol to examine the temporal aspects of Gq signaling in live cells. This methodology facilitates a nuanced understanding of Gq-mediated cellular responses, but also provides researchers with a valuable tool for dissecting other related signaling pathways.
本方案章节概述了使用生物发光共振能量转移 (BRET) 研究生长激素释放肽受体激活 Gq 信号的指南。本章节重点介绍 eBRET 和 NanoBRET™ 变体，涵盖了从细胞培养到转染、配体刺激和 BRET 测量的步骤，提供了一个强大的方案来研究活细胞中 Gq 信号的时间方面。该方法不仅有助于深入了解 Gq 介导的细胞反应，还为研究人员提供了一种有价值的工具，用于剖析其他相关信号通路。
