School of Biomedical Engineering, The University of Sydney, Camperdown, NSW, Australia.
School of Medicine, Western Sydney University, Campbelltown, NSW, Australia.
Methods Mol Biol. 2025;2861:89-96. doi: 10.1007/978-1-0716-4164-4_7.
Calcium imaging is a method that was first developed in the mid-1970s yet kept developing until current days to allow accurate measurement of free calcium ions in tissues. This widely used method has provided significant advances to our understanding of cellular signal transduction, including the discovery of subcellular compartmentalization of neurons and astrocytes, the identification of multiple signaling pathways, and mapping the functional connectivity between astrocytes and neuronal networks. Here we describe a method for the loading and imaging of cell-permeable AM ester calcium-sensitive dyes for the in vitro measurement of free intracellular Ca ions in acute brain slices.
钙成像技术是一种在上世纪 70 年代中期首次发展起来的方法，但直到今天仍在不断发展，以实现对组织中游离钙离子的精确测量。这种被广泛应用的方法为我们理解细胞信号转导提供了重要进展，包括发现神经元和星形胶质细胞的亚细胞区室化、确定多个信号通路以及绘制星形胶质细胞与神经元网络之间的功能连接。在这里，我们描述了一种用于加载和成像细胞通透性 AM 酯钙敏染料的方法，用于在体外测量急性脑切片中游离细胞内 Ca 离子。
