Drechsler H, Grunow R
Mol Gen Mikrobiol Virusol. 1985 Feb(2):30-3.
The mutations in the genes determining the levels of extracellular enzymes production were transferred into Bacillus subtilis 168 strain by genetic transformation technique. The method used has permitted registering the transfer of pleiotropic genes. Seven amylase producers were detected among 126 his+ transformants screened, as well as five metalprotease producers among 246 gly+ transformants. Cotransfer of pap and hpr mutant genes linked with his+ or gly+ genes might result in finding the producers among the mentioned prototrophic transformants. Selection of linked markers in transformation, presented in the paper, is discussed to be a useful technique for obtaining producer strains for industrial production.
通过遗传转化技术，将决定细胞外酶产生水平的基因突变导入枯草芽孢杆菌168菌株。所采用的方法能够记录多效性基因的转移。在筛选的126个组氨酸营养缺陷型（his+）转化子中检测到7个淀粉酶产生菌，在246个甘氨酸营养缺陷型（gly+）转化子中检测到5个金属蛋白酶产生菌。与his+或gly+基因连锁的pap和hpr突变基因的共转移可能会在上述原养型转化子中找到产生菌。本文介绍的转化中连锁标记的选择被认为是获得工业生产用生产菌株的一种有用技术。
