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从西番莲果皮水解物中生产木糖醇:水解和发酵过程的优化。

Xylitol production from passion fruit peel hydrolysate: Optimization of hydrolysis and fermentation processes.

机构信息

Federal University of Ceará, Department of Food Engineering, Center for Agricultural Sciences, Fortaleza, CE 60020-181, Brazil.

Federal University of Ceará, Department of Chemical Engineering, Technology Center, Fortaleza, CE 60455-760, Brazil.

出版信息

Bioresour Technol. 2024 Dec;414:131628. doi: 10.1016/j.biortech.2024.131628. Epub 2024 Oct 11.

Abstract

The passion fruit peel (PFP) has a high cellulose and hemicellulose content, which can be used to produce fermentable sugars. In this context, this study aims to optimize the release of xylose and the production of xylitol from PFP. The optimized conditions were 0.71 M dilute sulfuric acid and a 21.84-minute treatment, yielding 19.03 g/L of xylose (PFP-1). Different PFP hydrolysates were evaluated to improve xylitol production by the yeast Kluyveromyces marxianus ATCC 36907: PFP-2 (PFP1 treated with Ca(OH)), PFP-3 (PFP-1 treated with Ca(OH) and activated carbon), PFP-4 (PFP-3 with biological elimination of glucose with S. cerevisiae, and concentrated at different xylose concentrations). The applied methods resulted in higher xylitol production (14.97 g/L), when PFP hydrolysate was detoxified with Ca(OH), treated with activated charcoal for 1 h, biotreated for glucose removal, and concentrated to 40 g/L of xylose.

摘要

西番莲果皮(PFP)含有丰富的纤维素和半纤维素,可用于生产可发酵糖。在此背景下,本研究旨在优化西番莲果皮中木糖的释放和木糖醇的生产。优化条件为 0.71 M 稀硫酸,处理时间 21.84 分钟,可得到 19.03 g/L 的木糖(PFP-1)。评估了不同的西番莲果皮水解产物,以通过酵母 Kluyveromyces marxianus ATCC 36907 提高木糖醇的产量:PFP-2(用 Ca(OH)处理的 PFP1)、PFP-3(用 Ca(OH)和活性炭处理的 PFP-1)、PFP-4(用 S. cerevisiae 进行生物去除葡萄糖的 PFP-3,浓缩至不同浓度的木糖)。当用 Ca(OH)解毒、用活性炭处理 1 小时、生物去除葡萄糖并浓缩至 40 g/L 木糖的 PFP 水解产物时,应用这些方法可实现更高的木糖醇产量(14.97 g/L)。

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