在不同的抗衡离子中，合成 DNA 基序和双链 DNA 的电泳迁移率不同。
Differential electrophoretic mobility of synthetic DNA motifs and duplex DNA in various counter ions.
机构信息
The RNA Institute, University at Albany, State University of New York, New York, NY, USA.
Department of Nanoscale Science and Engineering, University at Albany, State University of New York, New York, NY, USA.
出版信息
Chem Commun (Camb). 2024 Oct 29;60(87):12706-12709. doi: 10.1039/d4cc04935g.
In this work, we analyzed the electrophoretic behavior of a double crossover (DX) DNA motif in various counter ions. The influence of the type and concentration of counter ions on electrophoretic behavior is different for the DX motif compared to a duplex of the same molecular weight. Higher concentrations of divalent ions Mg and Ca in the gel and running buffer reduce the electrophoretic migration of the DX motif. This effect is less pronounced in the monovalent ion Na while K ion did not have any significant effect on the electrophoretic behavior.
在这项工作中，我们分析了在不同反离子中双链交叉（DX）DNA 基序的电泳行为。与相同分子量的双链体相比，反离子的类型和浓度对 DX 基序的电泳行为的影响不同。凝胶和运行缓冲液中二价离子 Mg 和 Ca 的浓度较高会降低 DX 基序的电泳迁移率。在单价离子 Na 中，这种效应不太明显，而 K 离子对电泳行为没有任何显著影响。
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