The RNA Institute, University at Albany, State University of New York, Albany, New York 12222, United States.
ACS Appl Bio Mater. 2024 May 20;7(5):2704-2709. doi: 10.1021/acsabm.4c00274. Epub 2024 Apr 18.
The structural integrity, assembly yield, and biostability of DNA nanostructures are influenced by the metal ions used to construct them. Although high (>10 mM) concentrations of divalent ions are often preferred for assembling DNA nanostructures, the range of ion concentrations and the composition of the assembly products vary for different assembly conditions. Here, we examined the unique ability of Ba to retard double crossover DNA motifs by forming a low mobility species, whose mobility on the gel is determined by the concentration ratio of DNA and Ba. The formation of this electrophoretically retarded species is promoted by divalent ions such as Mg, Ca, and Sr when combined with Ba but not on their own, while monovalent ions such as Na, K, and Li do not have any effect on this phenomenon. Our results highlight the complex interplay between the metal ions and DNA self-assembly and could inform the design of DNA nanostructures for applications that expose them to multiple ions at high concentrations.
金属离子会影响 DNA 纳米结构的结构完整性、组装产率和生物稳定性。虽然通常需要使用高浓度（＞10mM）的二价离子来组装 DNA 纳米结构，但不同的组装条件下离子浓度范围和组装产物的组成会有所不同。在这里，我们研究了 Ba 形成低迁移率物种的独特能力，该物种通过形成低迁移率物种来延缓双交叉 DNA 基序，其在凝胶上的迁移率由 DNA 和 Ba 的浓度比决定。当与 Ba 结合时，Mg、Ca 和 Sr 等二价离子会促进这种电泳延迟物种的形成，但单独使用时则不会，而 Na、K 和 Li 等一价离子对这种现象没有任何影响。我们的研究结果强调了金属离子与 DNA 自组装之间的复杂相互作用，并为在高浓度下暴露于多种离子的 DNA 纳米结构的设计提供了信息。
