Abduh Muhammad Yusuf, Shafitri Tri Ramadianti, Elfahmi Elfahmi
School of Life Science and Technology, Institut Teknologi Bandung, Indonesia.
University Center of Excellence for Nutraceutical, Bioscience and Biotechnology Research Center, Institut Teknologi Bandung, Indonesia.
Heliyon. 2024 Sep 30;10(19):e38736. doi: 10.1016/j.heliyon.2024.e38736. eCollection 2024 Oct 15.
Propolis produced by stingless bees contains various chemical compounds that contribute to its bioactivity. The availability of certain plants at the growth site and the propolis's geographic origin have a significant impact on its chemical composition. The objective of this study was to examine the chemical profile, yield, total flavonoid and phenolic content, and antioxidant and anti-inflammatory effects of propolis extract from 10 distinct locations in Indonesia. The yield of propolis extract investigated in this study lies in the range of 26.25 ± 1.76 to 43.25 ± 3.60 %. The total phenolic content of the propolis extract varies from 50.03 ± 3.40-98.03 ± 13.94 mg GAE/g whereas the total flavonoid content of the propolis extract varies from and 0.70 ± 0.08-57.76 ± 0.67 mg QE/g. The antioxidant activity of the propolis extract in terms of IC values lies in the range of 332.07 ± 6.12 to 831.48 ± 29.48 ppm whereas the anti-inflammatory activity of the propolis extract in terms of IC values lies in the range of 28.69 ± 4.95 to 44.12 ± 19.22 ppm. Both the total flavonoid and phenolic content of the propolis extract from various locations were correlated with the antioxidant activities and anti-inflammatory activities. The results indicate that there was a significant negative correlation between the total flavonoid and phenolic content of the propolis extract with the antioxidant activity. However, the anti-inflammatory activity was not strongly correlated with the total flavonoid and phenolic content of the propolis extract. There were 36 volatile compounds in the propolis extract as identified by the Gas Chromatography-Mass Spectrometry with triterpenoid as the major substances (28.66-44.86 %). The presence of anti-inflammatory compounds particularly α, β-Amyrin (2.2-6.52 %) and lupeol (2-4.72 %) in the propolis extract highlights the potential of propolis in health and medicine application.
无刺蜂生产的蜂胶含有多种有助于其生物活性的化合物。生长地某些植物的可获得性以及蜂胶的地理来源对其化学成分有重大影响。本研究的目的是检测来自印度尼西亚10个不同地点的蜂胶提取物的化学特征、产量、总黄酮和酚类含量以及抗氧化和抗炎作用。本研究中所调查的蜂胶提取物产量在26.25±1.76%至43.25±3.60%的范围内。蜂胶提取物的总酚含量在50.03±3.40 - 98.03±13.94毫克没食子酸当量/克之间变化，而蜂胶提取物的总黄酮含量在0.70±0.08 - 57.76±0.67毫克槲皮素当量/克之间变化。蜂胶提取物的抗氧化活性以IC值计在332.07±6.12至831.48±29.48 ppm的范围内，而蜂胶提取物的抗炎活性以IC值计在28.69±4.95至44.12±19.22 ppm的范围内。来自不同地点的蜂胶提取物的总黄酮和酚类含量均与抗氧化活性和抗炎活性相关。结果表明，蜂胶提取物的总黄酮和酚类含量与抗氧化活性之间存在显著负相关。然而，抗炎活性与蜂胶提取物的总黄酮和酚类含量没有强烈相关性。通过气相色谱 - 质谱法鉴定出蜂胶提取物中有36种挥发性化合物，其中三萜类为主要物质（28.66 - 44.86%）。蜂胶提取物中存在抗炎化合物，特别是α，β - 香树脂醇（2.2 - 6.52%）和羽扇豆醇（2 - 4.72%），突出了蜂胶在健康和医学应用方面的潜力。
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