Abduh Muhammad Yusuf, Ramdhani Fahmi, Setiawan Albert, Rifqialdi Ghiffary, Rahmawati Anasya, Zainudin Ima Mulyama
School of Life Sciences and Technology, Institut Teknologi Bandung, Jalan Ganesha 10, 40132 Bandung, Indonesia.
University Center of Excellence for Nutraceuticals, Bioscience and Biotechnology Research Center, Institut Teknologi Bandung, Jalan Ganesha 10, 40132 Bandung, Indonesia.
Heliyon. 2023 Jun 17;9(6):e17304. doi: 10.1016/j.heliyon.2023.e17304. eCollection 2023 Jun.
This study investigated the effects of microclimate conditions on the activity of , , and cultivated in Modular Hives for producing crude propolis. The hives were equipped with sensors that recorded microclimate data within the hive as well as the total activity of bees entering and leaving the hives. This study also investigated the effects of cultivating , , and with resin towards the productivity of crude propolis and the effects of different extraction methods on the yield, total phenolic content, total flavonoid content, and antibacterial activity of propolis extract produced by the spp. Based on the statistical analysis, there is a significant positive correlation between temperature and light intensity towards the activity spp. entering and leaving the beehives. The productivity of crude propolis lies in the range of 1.22-5.88 g/colony/week whereas the yield of propolis extract varies from 15.12 to 24.17%. The total phenolic and flavonoid content of the propolis extract lies in the range of 123.81-343.93 mg GAE/g and 5.48-35.77 mg QE/g, respectively. The highest propolis yield (32.45 ± 0.90%) was obtained from the crude propolis produced by followed by Soxhlet extraction method. Propolis extract with the highest phenolic content (343.93 ± 44.32 mg GAE/g) and flavonoid content (35.77 ± 9.94 mg QE/g) was obtained from the propolis produced by followed by maceration method. All the propolis extracts inhibited the growth of with the inhibition diameter varies from 6.58 ± 0.04 mm to 9.70 ± 0.7 mm which be considered as moderate antimicrobial activity.
本研究调查了小气候条件对模块化蜂箱中用于生产粗蜂胶的[蜜蜂品种1]、[蜜蜂品种2]和[蜜蜂品种3]活动的影响。蜂箱配备了传感器，可记录蜂箱内的小气候数据以及进出蜂箱的蜜蜂的总活动情况。本研究还调查了用[树脂品种]树脂培育[蜜蜂品种1]、[蜜蜂品种2]和[蜜蜂品种3]对粗蜂胶生产力的影响，以及不同提取方法对[蜜蜂品种]生产的蜂胶提取物的产量、总酚含量、总黄酮含量和抗菌活性的影响。基于统计分析，温度和光照强度与进出蜂箱的[蜜蜂品种]活动之间存在显著正相关。粗蜂胶的生产力在1.22 - 5.88克/蜂群/周范围内，而蜂胶提取物的产量在从百分之15.12到百分之24.17之间变化。蜂胶提取物的总酚和黄酮含量分别在123.81 - 343.93毫克没食子酸当量/克和5.48 - 35.77毫克槲皮素当量/克范围内。从[蜜蜂品种]生产的粗蜂胶中获得了最高的蜂胶产量（百分之32.45 ± 0.90%），其次是索氏提取法。从[蜜蜂品种]生产的蜂胶中，采用浸渍法后获得了酚含量最高（343.93 ± 44.32毫克没食子酸当量/克）和黄酮含量最高（35.77 ± 9.94毫克槲皮素当量/克）的蜂胶提取物。所有蜂胶提取物均抑制了[细菌品种]的生长，抑菌直径在6.58 ± 0.04毫米至9.70 ± 0.7毫米之间，这被认为具有中等抗菌活性。
