Sun Shiyu, Chen Minghui, Zhang Tingting, Wang Yanyan, Shen Weijun, Zhang Tao, Liu Jian, Lan Haidan, Zhao Jianyuan, Lin Fuqing, Zhao Xuan
Department of Anesthesia, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai, 200072 China.
Institute for Developmental and Regenerative Cardiovascular Medicine, MOE-Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai, 200092 China.
Phenomics. 2024 Mar 10;4(3):227-233. doi: 10.1007/s43657-023-00123-z. eCollection 2024 Jun.
This research was to reveal the key factors in the progression of osteoarthritis (OA) using non-targeted metabolomics and to find targeted therapies for patients with OA. Twenty-two patients with knee OA scheduled for total knee arthroplasty were divided into two groups: Kellgren-Lawrence (KL) grade 3 ( = 16) and grade 4 ( = 6), according to plain X-rays of the knee. After the operation, the cartilages of femur samples were analyzed using non-targeted metabolomics. When compared with grade 3 patients, the levels of choline, 2-propylpiperidine, rhamnose, and monomethyl glutaric acid were higher; while 1-methylhistamine, sphingomyelin (SM) (d18:1/14:0), zeranol, 3- (4-hydroxyphenyl)-1-propanol, 5-aminopentanamide, dihydrouracil, 2-hydroxypyridine, and 3-amino-2-piperidone were lower in grade 4 patients. Furthermore, some metabolic pathways were found to be significantly different in two groups such as the pantothenate and coenzyme A (CoA) biosynthesis pathway, the glycerophospholipid metabolism pathway, histidine metabolism pathway, lysine degradation pathway, glycine, serine and threonine metabolism pathway, fructose and mannose metabolism pathway, the pyrimidine metabolism pathway, and beta-alanine metabolism pathway. This work used non-targeted metabolomics and screened out differential metabolites and metabolic pathways, providing a reliable theoretical basis for further study of specific markers and their specific pathways in the progression of OA.
The online version contains supplementary material available at 10.1007/s43657-023-00123-z.
本研究旨在利用非靶向代谢组学揭示骨关节炎（OA）进展的关键因素，并为OA患者寻找靶向治疗方法。根据膝关节X线平片，将22例计划行全膝关节置换术的膝骨关节炎患者分为两组：Kellgren-Lawrence（KL）3级（n = 16）和4级（n = 6）。术后，采用非靶向代谢组学分析股骨样本的软骨。与3级患者相比，4级患者的胆碱、2-丙基哌啶、鼠李糖和单甲基戊二酸水平较高；而1-甲基组胺、鞘磷脂（SM）（d18:1/14:0）、玉米赤霉醇、3-（4-羟苯基）-1-丙醇、5-氨基戊酰胺、二氢尿嘧啶、2-羟基吡啶和3-氨基-2-哌啶酮水平较低。此外，发现两组在一些代谢途径上存在显著差异，如泛酸和辅酶A（CoA）生物合成途径、甘油磷脂代谢途径、组氨酸代谢途径、赖氨酸降解途径、甘氨酸、丝氨酸和苏氨酸代谢途径、果糖和甘露糖代谢途径、嘧啶代谢途径和β-丙氨酸代谢途径。本研究利用非靶向代谢组学筛选出差异代谢物和代谢途径，为进一步研究OA进展中的特定标志物及其特定途径提供了可靠的理论依据。
在线版本包含可在10.1007/s43657-023-00123-z获取的补充材料。
