Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Nebraska Center for Integrated Biomolecular Communication, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Molecules. 2020 Nov 4;25(21):5128. doi: 10.3390/molecules25215128.
Nuclear Magnetic Resonance (NMR) spectroscopy is a quantitative analytical tool commonly utilized for metabolomics analysis. Quantitative NMR (qNMR) is a field of NMR spectroscopy dedicated to the measurement of analytes through signal intensity and its linear relationship with analyte concentration. Metabolomics-based NMR exploits this quantitative relationship to identify and measure biomarkers within complex biological samples such as serum, plasma, and urine. In this review of quantitative NMR-based metabolomics, the advancements and limitations of current techniques for metabolite quantification will be evaluated as well as the applications of qNMR in biomedical metabolomics. While qNMR is limited by sensitivity and dynamic range, the simple method development, minimal sample derivatization, and the simultaneous qualitative and quantitative information provide a unique landscape for biomedical metabolomics, which is not available to other techniques. Furthermore, the non-destructive nature of NMR-based metabolomics allows for multidimensional analysis of biomarkers that facilitates unambiguous assignment and quantification of metabolites in complex biofluids.
核磁共振（NMR）光谱学是一种常用于代谢组学分析的定量分析工具。定量核磁共振（qNMR）是核磁共振光谱学的一个领域，专门用于通过信号强度及其与分析物浓度的线性关系来测量分析物。基于代谢组学的 NMR 利用这种定量关系来识别和测量血清、血浆和尿液等复杂生物样本中的生物标志物。在本次基于定量 NMR 的代谢组学综述中，将评估当前代谢物定量技术的优缺点，以及 qNMR 在生物医学代谢组学中的应用。虽然 qNMR 受到灵敏度和动态范围的限制，但简单的方法开发、最小的样品衍生化以及同时提供定性和定量信息，为生物医学代谢组学提供了独特的视角，这是其他技术所不具备的。此外，基于 NMR 的代谢组学的非破坏性性质允许对生物标志物进行多维分析，这有助于在复杂生物流体中对代谢物进行明确的归属和定量。
