泻下多草药糊剂对洛哌丁胺诱导的大鼠便秘的影响。

Effect of laxative polyherbal paste for loperamide induced constipation in rats.

作者信息

Gull Imtiaz, Khan Imran Ahmad, Malik Abdul, Bukhari Malaika, Iqbal Muhammad Omer, Usman Muhammad, Hussain Kashif, Khan Maliha Khalid, Anwar Muhammad

机构信息

Fatima-Tu Zahra Department of Life Sciences, Muhammad Institute of Medical and Allied Sciences Multan 60000, Punjab, Pakistan.

Department of Pharmacy, MNS University of Agriculture Multan 60000, Punjab, Pakistan.

出版信息

Am J Transl Res. 2024 Sep 15;16(9):4714-4727. doi: 10.62347/XSFH4004. eCollection 2024.

DOI:10.62347/XSFH4004

PMID:39398568

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11470361/

Abstract

OBJECTIVE

The goal of this study was to find out if polyherbal paste (PHP) with , , and in honey could help rats that were constipated because of loperamide.

METHODS

Thirty male rats were divided into 6 groups: a control group receiving saline, a model group receiving loperamide at 10 mg/kg and saline, a phenolphthalein group (positive control) receiving loperamide at 10 mg/kg and phenolphthalein at 10 mg/kg, and low (20 mg/kg), medium (40 mg/kg), and high (60 mg/kg) doses of PHP, via intragastric administration for 7 days. Various parameters, including food consumption, water consumption, body weight, fecal characteristics, gastrointestinal transit rate, histological changes, serum biomarkers, and aquaporin-3 (AQP3) and C-kit protein expression levels, were assessed.

RESULTS

Administering PHP at a dose of 60 mg/kg resulted in a 16.89% increase in fecal water content, a 12.14% increase in the amount of feces, and a 23.67% increase in gastrointestinal transit rate, while also reducing the time to black stool and restoring appearance by 23.41%. At the 40 mg/kg dose, PHP increased motilin levels in the blood by 31.22%, gastrin by 52.78%, and substance P by 19.45% while decreasing somatostatin by 20.17%. Furthermore, at the 60 mg/kg dose, PHP decreased mucous membrane damage and goblet cell function in the colon, reduced AQP3 protein production by 33.39%, and increased c-kit protein production by 12.14%.

CONCLUSION

The PHP showed promising therapeutic potential for loperamide-induced constipation in rats.

摘要

目的

本研究的目的是探究含有[具体成分1]、[具体成分2]和[具体成分3]的蜂蜜多草药糊剂（PHP）是否有助于治疗因洛哌丁胺导致便秘的大鼠。

方法

将30只雄性大鼠分为6组：对照组接受生理盐水；模型组接受10 mg/kg洛哌丁胺和生理盐水；酚酞组（阳性对照组）接受10 mg/kg洛哌丁胺和10 mg/kg酚酞；低剂量（20 mg/kg）、中剂量（40 mg/kg）和高剂量（60 mg/kg）的PHP组，通过灌胃给药7天。评估了各种参数，包括食物摄入量、饮水量、体重、粪便特征、胃肠转运率、组织学变化、血清生物标志物以及水通道蛋白3（AQP3）和C-kit蛋白表达水平。

结果

以60 mg/kg剂量给予PHP可使粪便含水量增加16.89%，粪便量增加12.14%，胃肠转运率提高23.67%，同时还使黑便时间缩短23.41%并恢复外观。在40 mg/kg剂量下，PHP使血液中胃动素水平升高31.22%，胃泌素升高52.78%，P物质升高19.45%，而生长抑素降低20.17%。此外，在60 mg/kg剂量下，PHP减少了结肠黏膜损伤和杯状细胞功能，使AQP3蛋白产生减少33.39%，并使c-kit蛋白产生增加12.14%。