Gull Imtiaz, Khan Imran Ahmad, Malik Abdul, Bukhari Malaika, Iqbal Muhammad Omer, Usman Muhammad, Hussain Kashif, Khan Maliha Khalid, Anwar Muhammad
Fatima-Tu Zahra Department of Life Sciences, Muhammad Institute of Medical and Allied Sciences Multan 60000, Punjab, Pakistan.
Department of Pharmacy, MNS University of Agriculture Multan 60000, Punjab, Pakistan.
Am J Transl Res. 2024 Sep 15;16(9):4714-4727. doi: 10.62347/XSFH4004. eCollection 2024.
The goal of this study was to find out if polyherbal paste (PHP) with , , and in honey could help rats that were constipated because of loperamide.
Thirty male rats were divided into 6 groups: a control group receiving saline, a model group receiving loperamide at 10 mg/kg and saline, a phenolphthalein group (positive control) receiving loperamide at 10 mg/kg and phenolphthalein at 10 mg/kg, and low (20 mg/kg), medium (40 mg/kg), and high (60 mg/kg) doses of PHP, via intragastric administration for 7 days. Various parameters, including food consumption, water consumption, body weight, fecal characteristics, gastrointestinal transit rate, histological changes, serum biomarkers, and aquaporin-3 (AQP3) and C-kit protein expression levels, were assessed.
Administering PHP at a dose of 60 mg/kg resulted in a 16.89% increase in fecal water content, a 12.14% increase in the amount of feces, and a 23.67% increase in gastrointestinal transit rate, while also reducing the time to black stool and restoring appearance by 23.41%. At the 40 mg/kg dose, PHP increased motilin levels in the blood by 31.22%, gastrin by 52.78%, and substance P by 19.45% while decreasing somatostatin by 20.17%. Furthermore, at the 60 mg/kg dose, PHP decreased mucous membrane damage and goblet cell function in the colon, reduced AQP3 protein production by 33.39%, and increased c-kit protein production by 12.14%.
The PHP showed promising therapeutic potential for loperamide-induced constipation in rats.
本研究的目的是探究含有[具体成分1]、[具体成分2]和[具体成分3]的蜂蜜多草药糊剂（PHP）是否有助于治疗因洛哌丁胺导致便秘的大鼠。
将30只雄性大鼠分为6组：对照组接受生理盐水；模型组接受10 mg/kg洛哌丁胺和生理盐水；酚酞组（阳性对照组）接受10 mg/kg洛哌丁胺和10 mg/kg酚酞；低剂量（20 mg/kg）、中剂量（40 mg/kg）和高剂量（60 mg/kg）的PHP组，通过灌胃给药7天。评估了各种参数，包括食物摄入量、饮水量、体重、粪便特征、胃肠转运率、组织学变化、血清生物标志物以及水通道蛋白3（AQP3）和C-kit蛋白表达水平。
以60 mg/kg剂量给予PHP可使粪便含水量增加16.89%，粪便量增加12.14%，胃肠转运率提高23.67%，同时还使黑便时间缩短23.41%并恢复外观。在40 mg/kg剂量下，PHP使血液中胃动素水平升高31.22%，胃泌素升高52.78%，P物质升高19.45%，而生长抑素降低20.17%。此外，在60 mg/kg剂量下，PHP减少了结肠黏膜损伤和杯状细胞功能，使AQP3蛋白产生减少33.39%，并使c-kit蛋白产生增加12.14%。
PHP对大鼠洛哌丁胺诱导的便秘显示出有前景的治疗潜力。
