Ceccacci Sara, Lucia Adriana De, Tortora Assunta, Colantuono Antonio, Carotenuto Gennaro, Tito Annalisa, Monti Maria Chiara
Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy.
PhD Program in Drug Discovery and Development, Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy.
Antioxidants (Basel). 2022 Dec 6;11(12):2409. doi: 10.3390/antiox11122409.
Oxidative stress plays a major role in the skin aging process through the reactive oxygen species production and advanced glycation end products (AGEs) formation. Antioxidant ingredients are therefore needed in the skin care market and the use of molecules coming from plant cell cultures provide a unique opportunity. In this paper, the features of an hydroethanolic extract obtained by cells (JasHEx) were explored. The antioxidant and anti-AGE properties were investigated by a multidisciplinary approach combining mass spectrometric and bio-informatic in vitro and ex vivo experiments. JasHEx contains phenolic acid derivatives, lignans and triterpenes and it was found to reduce cytosolic reactive oxygen species production in keratinocytes exposed to exogenous stress. It also showed the ability to reduce AGE formation and to increase the collagen type I production in extracellular matrix. Data demonstrated that JasHEx antioxidant properties were related to its free radical scavenging and metal chelating activities and to the activation of the Nrf2/ARE pathway. This can well explain JasHEx anti-inflammatory activity related to the decrease in NO levels in LPS-stimulated macrophages. Thus, JasHEx can be considered a powerful antioxidant booster against oxidative stress-induced skin aging.
氧化应激通过活性氧的产生和晚期糖基化终产物(AGEs)的形成在皮肤衰老过程中起主要作用。因此,护肤品市场需要抗氧化成分,而使用来自植物细胞培养物的分子提供了一个独特的机会。在本文中,探索了通过细胞获得的水乙醇提取物(JasHEx)的特性。通过结合质谱和生物信息学的多学科方法,在体外和体内实验中研究了其抗氧化和抗AGE特性。JasHEx含有酚酸衍生物、木脂素和三萜类化合物,发现它能减少暴露于外源性应激的角质形成细胞中细胞溶质活性氧的产生。它还显示出减少AGE形成和增加细胞外基质中I型胶原蛋白产生的能力。数据表明,JasHEx的抗氧化特性与其自由基清除和金属螯合活性以及Nrf2/ARE途径的激活有关。这可以很好地解释JasHEx与LPS刺激的巨噬细胞中NO水平降低相关的抗炎活性。因此,JasHEx可被认为是一种强大的抗氧化增强剂,可对抗氧化应激诱导的皮肤衰老。