Wang Wei, Jiang Lina, Li Ran, Jiang Sai, Liu Yongcheng, Xing Jichun, Li Yujian
School of Life Sciences Qufu Normal University Qufu China.
Institute of Entomology Guizhou University Guiyang China.
Ecol Evol. 2024 Oct 11;14(10):e70413. doi: 10.1002/ece3.70413. eCollection 2024 Oct.
To delve deeper into the phylogenetic relationships within Cicadellidae and the taxonomic arrangement of Evacanthinae, our study focuses on the mitochondrial genome sequencing of five Nivanini species: , , , and . The results showed that the length of the five mitochondrial genomes ranged from 15,610 to 16,032 bp and included 37 typical genes. The A + T content of Nivanini ranged from 72.5% to 78.7%, which is consistent with that of other sequenced Evacanthinae species. All transfer RNAs (tRNAs) exhibit the typical cloverleaf secondary structure, except for (AGY), which lacks the dihydrouracil (DHU) arm. With regard to protein-coding genes, all started with ATN codons, except for , and most of them use TAA or TAG as termination codons. Using the Bayesian inference and maximum likelihood methods, a phylogenetic tree based on all 37 genes was constructed, with a total of 57 Cicadellidae species and two outgroups included as research objects. The analyses confirmed the monophyletic nature of each subfamily, highlighting Deltocephalinae as the oldest, distinctively parallel to the others.
为了更深入地探究叶蝉科内部的系统发育关系以及隐脉叶蝉亚科的分类安排，我们的研究聚焦于五种尼氏叶蝉属物种的线粒体基因组测序，即[物种1]、[物种2]、[物种3]、[物种4]和[物种5]。结果表明，这五个线粒体基因组的长度在15,610至16,032 bp之间，包含37个典型基因。尼氏叶蝉属的A + T含量在72.5%至78.7%之间，这与其他已测序的隐脉叶蝉亚科物种一致。所有转运RNA（tRNA）均呈现典型的三叶草二级结构，但[特定tRNA名称]（AGY）除外，它缺少二氢尿嘧啶（DHU）臂。关于蛋白质编码基因，除了[特定基因名称]外，所有基因均以ATN密码子起始，并且大多数基因使用TAA或TAG作为终止密码子。使用贝叶斯推断和最大似然法，基于所有这37个基因构建了系统发育树，总共纳入了57种叶蝉科物种和两个外群作为研究对象。分析证实了每个亚科的单系性质，突出显示叶蝉亚科是最古老的，与其他亚科明显平行。
