Institute of Entomology, The Provincial Key Laboratory for Agricultural Pest Management Mountainous Region, Guizhou University, Guiyang, Guizhou, China.
Shaanxi Key Laboratory of Bio-resources, Shaanxi University of Technology, Hanzhong, Shaanxi, China.
Arch Insect Biochem Physiol. 2022 Sep;111(1):e21902. doi: 10.1002/arch.21902. Epub 2022 Apr 11.
Ledrinae is a unique group of leafhoppers with a distinct appearance. Petalocephala is the largest Ledrinae genus that is difficult to identify except by dissecting the male genitals. To date, research on Ledrinae is relatively less compared with other leafhoppers. Therefore, to better understand this group, we sequenced and analyzed three complete Petalocephala mitochondrial genomes. We comparatively analyzed these general Petalocephala genomic features (including size, AT content, AT/GC skew, 13 protein-coding gene nucleotide compositions, etc.), and predicted 22 transfer RNA secondary structures. We obtained highly consistent phylogenetic results within Cicadellidae based on mitogenomic data using the maximum likelihood and Bayesian methods. Our results showed that all subfamilies were monophyletic and had a high node support rate, and there was a sister group relationship between Ledrinae and all other leafhopper groups. Furthermore, treehoppers were found to originate from leafhoppers and showed sister group relationships with Megophthalminae. Within Ledrinae, all phylogenetic trees supporting phylogenetic relationships were as follows: ([P. dicondylica + P. gongshanensis] + [Tituria pyramidata + [Ledra auditura + P. gongshanensis]]) Based on the complete mitogenome phylogenetic analysis and the comparison of morphological characteristics, we propose that Petalocephala is not monophyletic.
叶蝉是一类具有独特外观的叶蝉。花头叶蝉属是最大的叶蝉属，除了通过解剖雄性生殖器外，很难识别。迄今为止，与其他叶蝉相比，对花头叶蝉的研究相对较少。因此，为了更好地了解这一组，我们对三个完整的花头叶蝉线粒体基因组进行了测序和分析。我们比较分析了这些一般花头叶蝉基因组特征（包括大小、AT 含量、AT/GC 偏斜、13 个蛋白质编码基因核苷酸组成等），并预测了 22 个转移 RNA 二级结构。我们基于线粒体基因组数据使用最大似然法和贝叶斯法获得了高度一致的半翅目系统发育结果。我们的结果表明，所有亚科都是单系的，具有高节点支持率，花头叶蝉与所有其他叶蝉群具有姐妹群关系。此外，树蝉起源于叶蝉，与长头叶蝉科具有姐妹群关系。在花头叶蝉属中，所有支持系统发育关系的系统发育树如下：([P. dicondylica+P. gongshanensis]+[Tituria pyramidata+[Ledra auditura+P. gongshanensis]])。基于完整的线粒体基因组系统发育分析和形态特征比较，我们提出花头叶蝉属不是单系的。
