Jiang Sai, Jiang Lina, Li Ran, Gao Hui, Zhang Aijing, Yan Yurong, Zou Xinzhu, Wu Jixiao, Xu Shuying, Yi Xianfeng, Li Yujian
School of Life Sciences, Qufu Normal University, Qufu, China School of Life Sciences, Qufu Normal University Qufu China.
Biodivers Data J. 2022 Dec 9;10:e95935. doi: 10.3897/BDJ.10.e95935. eCollection 2022.
Lin et Li, 1990 belongs to Coleoptera, Curculionidae, Curculioninae, Anthonomini. It is a herbivorous insect that damages Blume (Ulmaceae) by affecting branch growth. The mitochondrial genome of was sequenced and annotated to better identify and related species. The total length of the mitochondrial genome was 17,071 bp, contained 37 typical genes (13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes) and two control regions (total length: 2,292 bp). Mitochondrial genome composition, nucleotide composition and codon usage are similar to those of other sequenced Curculionidae mitogenomes. All protein-coding genes initiated with ATN and TTG codons and ended with TAA, TAG or incomplete stop codons (TA, T). In addition, analyses of pairwise genetic distances between individual PCGs in Curculionidae species showed that ATP8 was the least conserved gene, while COI was the most conserved. Twenty-one transfer RNAs had typical cloverleaf structures, while trnS1 lacked dihydrouridine (DHU) arms. ML and BI analyses, based on 13 PCGs and two rRNAs from ten species of Curculionidae, strongly support the relationships between and species of the genus : ((+ ) + + (+ )) (BS = 100; PP = 1). Our phylogenetic analyses could mean that the genus should be sunk under , but more taxon sampling is needed to verify this result.
林氏象甲（Lin et Li, 1990）属于鞘翅目象甲科象甲亚科花象甲族。它是一种食草昆虫，通过影响树枝生长来危害朴属植物（榆科）。对其线粒体基因组进行了测序和注释，以便更好地识别该物种及相关物种。该线粒体基因组全长17,071 bp，包含37个典型基因（13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因）和两个控制区（全长：2,292 bp）。线粒体基因组组成、核苷酸组成和密码子使用情况与其他已测序的象甲科线粒体基因组相似。所有蛋白质编码基因均以ATN和TTG密码子起始，以TAA、TAG或不完整的终止密码子（TA、T）结束。此外，对象甲科物种中各个蛋白质编码基因之间的成对遗传距离分析表明，ATP8是最不保守的基因，而COI是最保守的。21个转运RNA具有典型的三叶草结构，而trnS1缺少二氢尿嘧啶（DHU）臂。基于象甲科10个物种的13个蛋白质编码基因和2个核糖体RNA进行的最大似然法（ML）和贝叶斯推断（BI）分析，有力地支持了林氏象甲与该属其他物种之间的关系：((+ ) + + (+ ))（自展支持率 = 100；后验概率 = 1）。我们的系统发育分析可能意味着林氏象甲属应归入 属之下，但需要更多的分类群采样来验证这一结果。
