Higher Institution Centre of Excellence, Borneo Marine Research Institute, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia.
Department of Marine Biology, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran.
PeerJ. 2024 Oct 10;12:e18149. doi: 10.7717/peerj.18149. eCollection 2024.
Marine invertebrates, including sponges, molluscs, jellyfish, mussels, and sea cucumbers, are abundant sources of high-quality collagen and offer advantages such as availability, ease of processing, lower inflammatory response, and good metabolic compatibility. Approximately 70% of the total protein in the body wall of sea cucumbers is collagen. Gelatin is a water-soluble protein produced from heat-denatured collagen and has various industrial applications.
Pepsin-solubilized collagen was extracted from the body wall of two sea cucumber and , species found in the Oman Sea and characterized with SDS-PAGE and amino acid composition. Then gelatin was extracted from pepsin-solubilized collagen of and some rheological properties were measured.
Amino acid composition and SDS-PAGE analysis showed that the collagen from both species was type I, with one α1 chain and β chains, with molecular weights of 125 and 250 kDa, respectively. Glycine was the most abundant amino acid in the collagen from both sea cucumber species. The pepsin-soluble collagens from both species had high levels of glycine, proline, alanine, glutamic acid, and hydroxyproline. The gelatin from had a melting point of 30 °C and displayed exceptional thermal stability, surpassing that of mammalian gelatin. Its gelling point was 5 °C, like that of cold-water fish gelatin, with a viscosity of 2.065 cp-lower than mammal gelatins. These findings suggested that collagen and gelatin from sea cucumbers could be useful in nutraceutical, pharmaceutical and cosmetic industries.
海洋无脊椎动物，包括海绵、软体动物、水母、贻贝和海参，是高质量胶原蛋白的丰富来源，具有可用性、易于加工、较低的炎症反应和良好的代谢相容性等优点。海参体壁的总蛋白质约有 70%是胶原蛋白。明胶是一种由热变性胶原蛋白产生的水溶性蛋白质，具有各种工业应用。
从阿曼海域发现的两种海参中提取胃蛋白酶溶解的胶原蛋白，并通过 SDS-PAGE 和氨基酸组成进行表征。然后从海参的胃蛋白酶溶解胶原蛋白中提取明胶，并测量一些流变性质。
氨基酸组成和 SDS-PAGE 分析表明，两种海参的胶原蛋白均为 I 型，具有一条α1 链和β链，分子量分别为 125 和 250 kDa。甘氨酸是两种海参胶原蛋白中最丰富的氨基酸。两种海参的胃蛋白酶可溶性胶原蛋白均具有高水平的甘氨酸、脯氨酸、丙氨酸、谷氨酸和羟脯氨酸。海参的明胶熔点为 30°C，具有出色的热稳定性，超过了哺乳动物明胶。其凝胶点为 5°C，与冷水鱼明胶相似，粘度为 2.065 cp，低于哺乳动物明胶。这些发现表明，海参胶原蛋白和明胶在营养保健品、制药和化妆品行业可能具有应用价值。
