State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Department of Statistics, Purdue University, West Lafayette, IN, 47907, USA.
Nat Commun. 2023 Oct 26;14(1):6813. doi: 10.1038/s41467-023-42608-5.
Shading in combination with extended photoperiods can cause exaggerated stem elongation (ESE) in soybean, leading to lodging and reduced yields when planted at high-density in high-latitude regions. However, the genetic basis of plant height in adaptation to these regions remains unclear. Here, through a genome-wide association study, we identify a plant height regulating gene on chromosome 13 (PH13) encoding a WD40 protein with three main haplotypes in natural populations. We find that an insertion of a Ty1/Copia-like retrotransposon in the haplotype 3 leads to a truncated PH13 with reduced interaction with GmCOP1s, resulting in accumulation of STF1/2, and reduced plant height. In addition, PH13 allele has been strongly selected for genetic improvement at high latitudes. Deletion of both PH13 and its paralogue PHP can prevent shade-induced ESE and allow high-density planting. This study provides insights into the mechanism of shade-resistance and offers potential solutions for breeding high-yielding soybean cultivar for high-latitude regions.
遮光和延长光照时间的组合会导致大豆茎伸长过度（ESE），当在高纬度地区高密度种植时，会导致倒伏和产量降低。然而，适应这些地区的植物高度的遗传基础仍不清楚。在这里，我们通过全基因组关联研究，在自然种群中鉴定出一个位于第 13 号染色体上的调节植物高度的基因（PH13），该基因编码一个 WD40 蛋白，具有三个主要单倍型。我们发现，单倍型 3 中的 Ty1/Copia 样逆转录转座子的插入导致 PH13 截断，与 GmCOP1s 的相互作用减少，导致 STF1/2 的积累，从而降低植物高度。此外，PH13 等位基因在高纬度地区的遗传改良中受到强烈选择。同时删除 PH13 和其同源基因 PHP 可以防止遮光引起的 ESE，并允许高密度种植。这项研究为耐荫性机制提供了新的见解，并为培育高纬度地区高产大豆品种提供了潜在的解决方案。
