Sun Maolin, Zhao Tianxin, Liu Shuang, Han Jinfeng, Wang Yuhe, Zhao Xue, Li Yongguang, Teng Weili, Zhan Yuhang, Han Yingpeng
Key Laboratory of Soybean Biology in Chinese Ministry of Education (Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry), Northeast Agricultural University, Harbin 150030, China.
Plants (Basel). 2024 Aug 16;13(16):2283. doi: 10.3390/plants13162283.
The utilization of saline land is a global challenge, and cultivating salt-tolerant soybean varieties is beneficial for improving the efficiency of saline land utilization. Exploring the genetic basis of salt-tolerant soybean varieties and developing salt-tolerant molecular markers can effectively promote the process of soybean salt-tolerant breeding. In the study, the membership function method was used to evaluate seven traits related to salt tolerance and comprehensive salt tolerance at the soybean seedling stage; genome-wide association analysis (GWAS) was performed in a natural population containing 200 soybean materials; and linkage analysis was performed in 112 recombinant inbred lines (RIL) population to detect quantitative trait loci (QTLs) of salt tolerance. In the GWAS, 147 SNPs were mapped, explaining 5.28-17.16% of phenotypic variation. In the linkage analysis, 10 QTLs were identified, which could explain 6.9-16.16% of phenotypic variation. And it was found that there were two co-located regions between the natural population and the RIL population, containing seven candidate genes of salt tolerance in soybean. In addition, one colocalization interval was found to contain qZJS-15-1, rs47665107, and rs4793412, all of which could explain more than 10% of phenotypic variation rates, making it suitable for molecular marker development. The physical positions of rs47665107 and rs47934112 were included in qZJS-15-1. Therefore, a KASP marker was designed and developed using Chr. 15:47907445, which was closely linked to the qZJS-15-1. This marker could accurately and clearly cluster the materials of salt-tolerant genotypes in the heterozygous population tested. The QTLs and KASP markers found in the study provide a theoretical and technical basis for accelerating the salt-tolerant breeding of soybean.
盐碱地的利用是一项全球性挑战，培育耐盐大豆品种有利于提高盐碱地利用效率。探索耐盐大豆品种的遗传基础并开发耐盐分子标记能够有效推动大豆耐盐育种进程。在本研究中，采用隶属函数法对大豆苗期与耐盐性相关的7个性状及综合耐盐性进行评价；在包含200份大豆材料的自然群体中进行全基因组关联分析（GWAS）；并在112个重组自交系（RIL）群体中进行连锁分析以检测耐盐性数量性状位点（QTL）。在GWAS中，定位了147个单核苷酸多态性（SNP），解释了5.28%-17.16%的表型变异。在连锁分析中，鉴定出10个QTL，可解释6.9%-16.16%的表型变异。并且发现自然群体和RIL群体之间存在两个共定位区域，包含大豆中7个耐盐候选基因。此外，发现一个共定位区间包含qZJS-15-1、rs47665107和rs4793412，它们均能解释超过10%的表型变异率，适合用于分子标记开发。rs47665107和rs47934112的物理位置包含在qZJS-15-1中。因此，利用Chr. 15:47907445设计并开发了一个竞争性等位基因特异性PCR（KASP）标记，其与qZJS-15-1紧密连锁。该标记能够准确清晰地对测试杂合群体中耐盐基因型材料进行聚类。本研究中发现的QTL和KASP标记为加速大豆耐盐育种提供了理论和技术基础。
