Forghani Nafiseh, Hosseinian Sara, Akhoond-Ali Zahra, Gholami Arman Abroumand, Assaran-Darban Reza, Vafaee Farzaneh
Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Res Pharm Sci. 2024 Aug 19;19(4):436-446. doi: 10.4103/RPS.RPS_24_23. eCollection 2024 Aug.
Stress, especially immobility stress, is quite common and one of the most important and influential risk factors in neurological disorders. This study aimed to investigate the effect of acute and chronic immobility stress on the level of cortical and hippocampal oxidative stress indicators and memory impairment following global cerebral ischemia.
In this study, 48 male Wistar rats were randomly divided into 6 groups: 1, sham (S); 2, sham-acute stress (SSA); 3, sham-chronic stress (SSC); 4, ischemia (IS); 5, ischemia-acute stress (ISA); 6, ischemia-chronic stress (ISC). The Morris water maze (MWM) test was performed 14 days after surgery, and cortisol levels and oxidative stress factors such as malondialdehyde MDA and total thiol were measured.
FINDINGS/RESULTS: In the MWM test, the time to find the platform (latency time) in the ISC and IS groups significantly increased compared to the S group. The time spent in the target quarter in these two groups was significantly reduced compared to the S group on the day of the probe. The results showed a significant increase in cortisol levels and malondialdehyde concentration in the ISA, ISC, and IS groups compared to the S group, but there was no significant difference in total thiol concentration. No significant difference was observed in the level of oxidative stress factors in the cortex.
Chronic immobility stress could reduce antioxidant factors in the hippocampus and exacerbate memory impairment caused by global ischemia.
应激，尤其是制动应激，十分常见，是神经疾病中最重要且最具影响力的风险因素之一。本研究旨在探讨急性和慢性制动应激对全脑缺血后皮质和海马氧化应激指标水平及记忆损伤的影响。
在本研究中，48只雄性Wistar大鼠被随机分为6组：1，假手术组（S）；2，假手术-急性应激组（SSA）；3，假手术-慢性应激组（SSC）；4，缺血组（IS）；5，缺血-急性应激组（ISA）；6，缺血-慢性应激组（ISC）。术后14天进行莫里斯水迷宫（MWM）试验，并检测皮质醇水平以及丙二醛（MDA）和总巯基等氧化应激因子。
在MWM试验中，ISC组和IS组找到平台的时间（潜伏期）相较于S组显著增加。在探测日，这两组在目标象限所花费的时间相较于S组显著减少。结果显示，与S组相比，ISA组、ISC组和IS组的皮质醇水平和丙二醛浓度显著升高，但总巯基浓度无显著差异。皮质中氧化应激因子水平未观察到显著差异。
慢性制动应激会降低海马中的抗氧化因子，并加剧全脑缺血所致的记忆损伤。
