Alipourfard Fatemeh, Shajiee Hooman, Nazari-Serenjeh Farzaneh, Hojati Vida, Alirezaie Masoud
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Department of Biology, Payame Noor University (PNU), Tehran, Iran.
Res Pharm Sci. 2023 Mar 10;18(3):270-278. doi: 10.4103/1735-5362.371583. eCollection 2023 May-Jun.
Increasing evidence indicates that oxidative stress is an important factor in the pathogenesis and progression of Alzheimer's disease (AD). Betaine is trimethylglycine with antioxidant and neuroprotective properties. The present study aimed to evaluate the possible beneficial effects of betaine on oxidative stress and memory deficits induced by intrahippocampal injection of amyloid beta (Aß) in an AD model.
Forty adult male Wistar rats were divided into 5 equal groups: the control and Aß groups which received oral gavage of saline (1 mL daily) for 14 days. The other 3 groups (betaine + Aß) received betaine (5, 10, and 15 mg/kg, orally) for 14 consecutive days. On the 15 day, all of the groups were injected bilaterallyintrahippocampal of Aß (5 µg/µL), except controls that were injected with normal saline as a vehicle. Seven days after the Aß injection, memory was assessed in a passive avoidance test. Changes in catalase activities and glutathione peroxidase, glutathione, and malondialdehyde concentrations were investigated to determine the antioxidant activity in the rat hippocampus.
FINDINGS/RESULTS: Data showed that betaine pretreatment of Aß-injected rats improved memory in avoidance tasks. In addition, betaine pretreatment attenuated oxidative stress.
The current findings showed that oral administration of betaine could prevent Aß-induced impairment of memory possibly through suppression of oxidative stress in the hippocampus area of rats.
越来越多的证据表明，氧化应激是阿尔茨海默病（AD）发病机制和病情进展中的一个重要因素。甜菜碱是具有抗氧化和神经保护特性的三甲基甘氨酸。本研究旨在评估甜菜碱对AD模型中海马内注射β淀粉样蛋白（Aβ）所诱导的氧化应激和记忆缺陷可能产生的有益作用。
40只成年雄性Wistar大鼠被分为5组，每组数量相等：对照组和Aβ组，连续14天每天经口灌胃给予生理盐水（1 mL）。另外3组（甜菜碱+Aβ组）连续14天每天经口给予甜菜碱（5、10和15 mg/kg）。在第15天，除了对照组注射生理盐水作为溶剂外，所有组均双侧海马内注射Aβ（5 μg/μL）。Aβ注射7天后，通过被动回避试验评估记忆。检测过氧化氢酶活性以及谷胱甘肽过氧化物酶、谷胱甘肽和丙二醛浓度的变化，以确定大鼠海马中的抗氧化活性。
数据显示，对注射Aβ的大鼠进行甜菜碱预处理可改善其在回避任务中的记忆。此外，甜菜碱预处理减轻了氧化应激。
目前的研究结果表明，口服甜菜碱可能通过抑制大鼠海马区的氧化应激来预防Aβ诱导的记忆损害。
