Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
J Phys Chem B. 2024 Oct 24;128(42):10311-10328. doi: 10.1021/acs.jpcb.4c02746. Epub 2024 Oct 14.
Recent advances in microfluidic technology have made it possible to image live bacterial cells with a high degree of precision and control. In particular, single-cell microfluidic designs have created new opportunities to study phenotypic variation in bacterial populations. However, the development and use of microfluidic devices require specialized resources, and these can be practical barriers to entry for microbiologists. With this review, our intentions are to help demystify the design, construction, and application of microfluidics. Our approach is to present design elements as building blocks from which a multitude of microfluidics applications can be imagined by the microbiologist.
微流控技术的最新进展使得对活细菌细胞进行高精度和高可控性成像成为可能。特别是，单细胞微流控设计为研究细菌群体中的表型变异创造了新的机会。然而，微流控器件的开发和使用需要专门的资源，这可能成为微生物学家进入的实际障碍。有鉴于此，我们旨在帮助大家揭开微流控设计、构建和应用的神秘面纱。我们的方法是将设计元素作为构建模块呈现出来，微生物学家可以根据这些模块想象出多种微流控应用。
