Mohammadzadeh Nima, Razavi Shabnam, Ebrahimipour Gholamhossein
Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
AMB Express. 2024 Oct 14;14(1):115. doi: 10.1186/s13568-024-01769-2.
Bariatric surgery is vital for sustainable weight loss and metabolic improvement in obese individuals, but its effects on gut microbiota and their role in these benefits require further investigation. Investigate the temporal changes in gut microbiota in obese patients undergoing bariatric surgery (gastric sleeve gastrectomy or Roux-en-Y Gastric Bypass (RYGB)) compared to healthy controls, aiming to understand their role in weight loss and metabolic health improvement. A case-control study included 30 obese patients aged 65-95 undergoing bariatric surgery, and 18 matched healthy controls. Selection criteria were based on age, race, BMI, history of antibiotics, probiotics, and prebiotics usage. Stool samples were collected at baseline, three months, and six months post-surgery for DNA extraction and quantitative real-time PCR analysis to assess gut microbiota changes. Physical activity and dietary intake were evaluated using standardized questionnaires. Statistical analyses were performed using R. Post-surgery, patients showed significant reductions in weight and BMI, with changes in dietary habits and physical activity. Quantitative real-time PCR analysis revealed substantial alterations in bacterial groups such as Bacteroides and Fusobacterium. However, some groups showed no significant changes, indicating a complex interaction between gut microbiota and bariatric surgery. Notable correlations were found between body weight, BMI, and specific bacterial groups like the C. cluster IV and Lactobacillus, particularly in RYGB patients. Bariatric surgery significantly alters gut microbiota, aiding weight loss and metabolic regulation in obese patients. Understanding these changes is crucial for developing effective obesity management strategies, requiring further research to optimize outcomes.
减重手术对于肥胖个体的可持续体重减轻和代谢改善至关重要，但其对肠道微生物群的影响以及它们在这些益处中所起的作用仍需进一步研究。研究接受减重手术（袖状胃切除术或Roux-en-Y胃旁路术（RYGB））的肥胖患者与健康对照相比肠道微生物群的时间变化，旨在了解它们在体重减轻和代谢健康改善中的作用。一项病例对照研究纳入了30名年龄在65 - 95岁接受减重手术的肥胖患者和18名匹配的健康对照。选择标准基于年龄、种族、体重指数、抗生素使用史、益生菌和益生元使用情况。在基线、术后三个月和六个月收集粪便样本进行DNA提取和定量实时PCR分析，以评估肠道微生物群的变化。使用标准化问卷评估身体活动和饮食摄入情况。使用R进行统计分析。术后，患者体重和体重指数显著降低，饮食习惯和身体活动也有所改变。定量实时PCR分析显示拟杆菌属和梭杆菌属等细菌类群有显著变化。然而，一些类群没有显著变化，表明肠道微生物群与减重手术之间存在复杂的相互作用。在体重、体重指数与特定细菌类群如IV群梭菌属和乳酸杆菌属之间发现了显著相关性，尤其是在RYGB患者中。减重手术显著改变肠道微生物群，有助于肥胖患者减轻体重和调节代谢。了解这些变化对于制定有效的肥胖管理策略至关重要，需要进一步研究以优化治疗效果。
