Komissarov N S, Dyakov M Yu, Garibova L V
Moscow State University, Moscow, Russia.
Dokl Biol Sci. 2024 Dec;519(1):387-396. doi: 10.1134/S0012496624701278. Epub 2024 Oct 11.
The work contains a comparative analysis of methods for storing pure cultures of macrofungi. The study used 20 species of macrofungi from various taxonomic and ecological-trophic groups. Storage was carried out using five methods: serial subculturing, storage under a layer of distilled water and three cryopreservation protocols: a protocol using blocks of agar medium, a "perlite protocol," and a "grain protocol." For the selected cryostorage methods, various cryoprotective compounds (glycerol, trehalose) were used. Radial growth rate was used as a criterion for the state of crops. The values of the radial growth rate obtained immediately after isolation of the pure culture were chosen as the control. It has been shown that the most favorable for preserving the physiological activity of cultures are the storage method under a layer of distilled water, "perlite" and "grain" cryopreservation protocols.
该研究对大型真菌纯培养物的保存方法进行了比较分析。研究使用了20种来自不同分类和生态营养类群的大型真菌。采用了五种保存方法：连续继代培养、在蒸馏水层下保存以及三种冷冻保存方案：使用琼脂培养基块的方案、“珍珠岩方案”和“谷粒方案”。对于选定的冷冻保存方法，使用了各种冷冻保护化合物（甘油、海藻糖）。以径向生长速率作为衡量培养物状态的标准。将纯培养物分离后立即获得的径向生长速率值作为对照。结果表明，最有利于保持培养物生理活性的是在蒸馏水层下保存、“珍珠岩”和“谷粒”冷冻保存方案。
