Chernova O A, Kayumov A R, Markelova M I, Salnikov V V, Kutyreva M P, Khannanov A A, Fedorova M S, Zhuravleva D E, Baranova N B, Faizullin D A, Zuev Yu F, Chernov V M
Kazan Institute of Biochemistry and Biophysics, Federal Research Center Kazan Scientific Center of Russian Academy of Sciences, Kazan, Russia.
Kazan Federal University, Kazan, Russia.
Dokl Biol Sci. 2024 Dec;519(1):295-300. doi: 10.1134/S0012496624600246. Epub 2024 Oct 11.
Significant changes in lactobacillus-derived extracellular membrane vesicles, which transfer lipids, polysaccharides, proteins, and nucleic acids, were for the first time observed to accompany the development of resistance to antibiotics (amoxicillin and clarithromycin) in vitro in the probiotic strain Lactiplantibacillus plantarum 8p-a3. The changes occur together with large-scale genome rearrangements, changes in the profile of phenotypic sensitivity to antimicrobials of various groups, and evolution of virulence. Changes in vesicles affected their structure, composition, and activity against biofilms of opportunistic bacteria. The data provide for a better understanding of the molecular mechanisms of microbial survival under selective pressure of antimicrobials, the functional potential of probiotic vesicles, and probiotic safety assessments.
首次观察到，在体外培养益生菌植物乳杆菌8p-a3对抗生素（阿莫西林和克拉霉素）产生耐药性的过程中，源自乳酸杆菌的细胞外膜囊泡发生了显著变化，这些囊泡可转运脂质、多糖、蛋白质和核酸。这些变化与大规模基因组重排、对各类抗菌药物的表型敏感性谱变化以及毒力进化同时发生。囊泡的变化影响了它们的结构、组成以及对机会性细菌生物膜的活性。这些数据有助于更好地理解微生物在抗菌药物选择压力下的生存分子机制、益生菌囊泡的功能潜力以及益生菌安全性评估。
