College of Life Sciences, Hebei University, Baoding 071002, Hebei Province, PR China.
Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding 071002, Hebei Province, PR China.
Int J Syst Evol Microbiol. 2024 Oct;74(10). doi: 10.1099/ijsem.0.006543.
A novel actinomycetes producing lycopene, designated HBU208002, was isolated from a lakeside soil sample collected in Baiyangdian, located in Xiong'an New Area of China, and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that the strain HBU208002 fell within the genus and was closely related to JCM 14904 (99.73% identity). However, the average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH) and average amino acid identity (AAI) values between the strain HBU208002 and JCM 14904 were 91.78, 44.7 and 91.6%, respectively, which were lower than the ANI (95-96%), DDH (>70%) and AAI (>95%) thresholds of prokaryotic microbial defined species. The predominant fatty acids of the strain HBU208002 were iso-C, C ω8c. The menaquinones of the strain HBU208002 were MK-8(H) and MK9(H), while those of JCM 14904 were MK-10(H), MK-10(H), MK-9(H) and MK-9(H). Meanwhile, some phenotypic characterizations and antibacterial activities distinguished the strain HBU208002 from JCM 14904. The strain HBU208002 exhibited inhibitory effects on , and , but JCM 14904 had no activity. Therefore, the strain HBU208002 should be assigned as representing a novel species of the genus , for which the name was proposed. The type strain is HBU208002 (=KCTC49833 = GDMCC4.236).
一株产番茄红素的放线菌 HBU208002 是从中国雄安新区白洋淀湖边土壤中分离得到的，采用多相分类法对其分类地位进行了研究。基于 16S rRNA 基因序列比较的系统发育分析表明，该菌株 HBU208002 属于 属，与 JCM 14904 （99.73%同源性）最为密切。然而，菌株 HBU208002 与 JCM 14904 之间的平均核苷酸同一性（ANI）、数字 DNA-DNA 杂交（dDDH）和平均氨基酸同一性（AAI）值分别为 91.78%、44.7%和 91.6%，低于原核微生物定义种的 ANI（95-96%）、dDDH（>70%）和 AAI（>95%）的阈值。菌株 HBU208002 的主要脂肪酸为 iso-C 和 C ω8c。菌株 HBU208002 的menaquinones 为 MK-8(H)和 MK9(H)，而 JCM 14904 的menaquinones 为 MK-10(H)、MK-10(H)、MK-9(H)和 MK-9(H)。同时，一些表型特征和抗菌活性将菌株 HBU208002 与 JCM 14904 区分开来。菌株 HBU208002 对 、 和 表现出抑制作用，但 JCM 14904 没有活性。因此，菌株 HBU208002 应被鉴定为代表 属的一个新种，命名为 。其模式菌株为 HBU208002（=KCTC49833=GDMCC4.236）。
