CMET, Universiteit Gent, Ghent, Belgium.
EMS Laboratory, Section of Biological and Chemical Engineering (BCE), Department of Engineering, Aarhus University, Aarhus, Denmark.
Environ Microbiol. 2024 Oct;26(10):e16698. doi: 10.1111/1462-2920.16698.
Recently, there has been increased attention to hydrocarbonoclastic bacteria in the plastisphere. One particular genus, Alcanivorax, is reported in the biodegradation of several polymers in the literature. In this study, we further explored the role of Alcanivorax in the early colonization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH), nylon 6/69, and a novel plastic B4PF01. Starting from enrichments of a one-year experiment with a maximum relative abundance of 58.8% of Alcanivorax, two parallel experiments were set up. One experiment followed growth and activity during the first 21 days of plastic incubations, and the other followed the same parameters on the different material fractions of the plastics, such as leachables and pure polymer. For all plastic types, the highest microbial growth was associated with the total plastics compared to the other material fractions. A relative abundance of 62.7% of Alcanivorax in the nylon 6/69-enriched community was observed. This, combined with data on activity, suggests that nylon 6/69 is potentially degraded by this genus. Two isolates were obtained, closely related to A. borkumensis SK2 and Alcanivorax sp. DG881. The activity and growth of the isolates as axenic cultures resemble their abundance in the community. In conclusion, this study contributes to the knowledge of the role of Alcanivorax in plastic-enriched communities.
最近，人们对塑料圈内的烃类降解菌越来越关注。在文献中，有报道称特别是 Alcanivorax 属的细菌参与了几种聚合物的生物降解。在这项研究中，我们进一步探索了 Alcanivorax 在聚(3-羟基丁酸-co-3-羟基己酸)(PHBH)、尼龙 6/69 和新型塑料 B4PF01 早期定殖中的作用。从为期一年的实验中进行了富集，其中 Alcanivorax 的相对丰度最高可达 58.8%，随后进行了两个平行实验。一个实验跟踪了塑料培养前 21 天的生长和活性，另一个实验则跟踪了不同塑料材料（如可浸出物和纯聚合物）的相同参数。对于所有类型的塑料，与其他材料相比，总塑料与微生物生长的相关性最高。在尼龙 6/69 富集群落中观察到 Alcanivorax 的相对丰度为 62.7%。这与活性数据结合表明，尼龙 6/69 可能被该属降解。获得了两个分离株，与 A. borkumensis SK2 和 Alcanivorax sp. DG881 密切相关。分离株作为无菌培养物的活性和生长与其在群落中的丰度相似。总之，本研究有助于了解 Alcanivorax 在富含塑料的群落中的作用。
