Tiwari Viral Kumar, Vishwakarma Akanksha, Verma Digvijay, Sharma Monica
Department of Biotechnology, School of Life Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh, 226025, India.
Department of Environmental Microbiology, School of Earth and Environmental Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh, 226025, India.
World J Microbiol Biotechnol. 2025 Jun 3;41(6):187. doi: 10.1007/s11274-025-04404-9.
Municipal solid waste landfills are rich in plastic waste, fostering a unique microbial ecosystem distinct from natural habitats. This study aimed to investigate the microbial diversity at four landfill and plastic dump sites using a 16S rDNA metataxanomics approach, and to predict their potential for plastic degradation. Environmental DNA was isolated and analyzed through Illumina MiSeq sequencing. The results revealed a dominant presence of Pseudomonadota (47%) across all sites, with key genera including Streptomyces, Galbibacter, Alcanivorax, and OM190. The most abundant species were unclassified Chloroflexi bacterium, Galbibacter marinus, Gracilimonas amylolytica, and Teredinibacter sp. Alpha diversity analysis showed the highest species richness in plastic dump site in Utrathia (Lucknow), followed by Ghazipur landfill Site (Delhi), with low evenness across the bacterial communities. Beta diversity analysis, using Bray-Curtis and PCA, indicated distinct microbial profiles for each site. The PICRUSt analysis identified 402 genes related to nine enzyme categories involved in plastic degradation. Pearson correlation network analysis of top 1% genera highlighted positive associations between genera like Galbibacter, Alcanivorax, Thioalkalimicrobium, Idiomarina and Pseudomonas with peroxygenase enzyme. This study underscores the microbial diversity and functional potential of landfill microbes in plastic degradation, contributing to our understanding of the plastisphere microbiome in landfill environments.
城市固体垃圾填埋场富含塑料垃圾，形成了一个与自然栖息地不同的独特微生物生态系统。本研究旨在采用16S rDNA宏分类学方法调查四个垃圾填埋场和塑料倾倒场的微生物多样性，并预测它们降解塑料的潜力。通过Illumina MiSeq测序对环境DNA进行分离和分析。结果显示，所有场地中变形菌门（47%）占主导地位，关键属包括链霉菌属、海栖菌属、食烷菌属和OM190。最丰富的物种是未分类的绿弯菌门细菌、海栖海栖菌、解淀粉纤细单胞菌和船蛆杆菌属。α多样性分析表明，乌特拉希亚（勒克瑙）的塑料倾倒场物种丰富度最高，其次是加济布尔垃圾填埋场（德里），细菌群落的均匀度较低。使用Bray-Curtis和主成分分析（PCA）的β多样性分析表明，每个场地的微生物谱不同。PICRUSt分析确定了402个与参与塑料降解的九种酶类别相关的基因。对前1%的属进行的Pearson相关网络分析突出了海栖菌属、食烷菌属、硫碱微菌属、嗜压菌属和假单胞菌属等属与过氧酶之间的正相关关系。本研究强调了垃圾填埋场微生物在塑料降解方面的微生物多样性和功能潜力，有助于我们了解垃圾填埋场环境中的塑料球微生物群。
