College of Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, China.
College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Sci Rep. 2024 Oct 14;14(1):24033. doi: 10.1038/s41598-024-75146-1.
The leafminer Liriomyza trifolii causes severe economic damage on ornamental and horticultural crops in China. Rapid cold hardening (RCH) is a phenomenon where cold tolerance in insects can be significantly enhanced after a short-term acclimation to low temperatures. In this study, the regulation of transcription in response to cold hardening was investigated in L. trifolii adults, and fatty acids and cryoprotectant levels were measured. The composition of fatty acids changed after RCH treatment, and glucose and trehalose levels showed significant accumulation after acclimation, thus indicating that changes in fatty acids and cryoprotectants contribute to RCH in L. trifolii. RNA-seq was used to analyze transcriptional regulation after a 4 h hardening period and showed that differentially expressed genes clustered in multiple metabolic pathways, which indicates the importance of transcriptional regulation in RCH. This study expands our knowledge of biochemical and transcriptional changes in L. trifolii during cold hardening and provides a basis for further investigations aimed at understanding thermal adaptation in insects.
潜叶蝇 Liriomyza trifolii 对中国观赏园艺作物造成严重经济损失。快速冷驯化（RCH）是一种现象，昆虫的耐寒性可以在短期适应低温后显著增强。在这项研究中，研究了 L. trifolii 成虫对冷驯化的转录调控，并测量了脂肪酸和抗冻保护剂的水平。脂肪酸的组成在 RCH 处理后发生变化，葡萄糖和海藻糖水平在适应后表现出明显的积累，这表明脂肪酸和抗冻保护剂的变化有助于 L. trifolii 的 RCH。RNA-seq 用于分析 4 小时硬化期后的转录调控，结果表明差异表达基因聚类在多个代谢途径中，这表明转录调控在 RCH 中的重要性。本研究扩展了我们对 L. trifolii 冷驯化过程中生化和转录变化的认识，并为进一步研究昆虫的热适应提供了基础。
