Hubei Key Laboratory of Biologic Resources Protection and Utilization, Hubei Minzu University, Enshi, 445000, P. R. China.
School of Biological Science and Technology, Hubei Minzu University, Enshi, 445000, P. R. China.
BMC Plant Biol. 2024 Oct 15;24(1):965. doi: 10.1186/s12870-024-05668-3.
Glechoma longituba, recognized as a medicinal plant, provides valuable pharmaceutical raw materials for treating various diseases. Saline-alkali stress may effectively enhance the medicinal quality of G. longituba by promoting the synthesis of secondary metabolites. To investigate the changes in the primary medicinal components of G. longituba under saline-alkali stress and improve the quality of medicinal materials, NaCO was applied to induce short-term stress under different conditions and the biomass, physiologically active substances and primary medicinal components of G. longituba were measured in this study.
Under alkaline salt stress, the activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) were elevated in G. longituba, accompanied by increased accumulation of proline (Pro) and malondialdehyde (MDA). Furthermore, analysis of the medicinal constituents revealed that G. longituba produced the highest levels of soluble sugars, flavonoids, ursolic acid, and oleanolic acid under 0.6% NaCO stress for 48 h, 0.2% NaCO stress for 72 h, 0.4% NaCO stress for 12 h, and 0.4% NaCO stress for 8 h, respectively.
Short-term NaCO stress enhances the synthesis of medicinal components in G. longituba. By manipulating stress conditions, the production of various medicinal substances could be optimized. This approach may serve as a basis for the targeted cultivation of G. longituba, offering potential applications in the treatment of diverse diseases.
蔊菜作为一种药用植物，为治疗各种疾病提供了有价值的药物原料。盐碱性胁迫通过促进次生代谢产物的合成，可有效提高蔊菜的药用品质。为了研究盐碱性胁迫对蔊菜主要药用成分的变化，提高药材质量，本研究采用 NaCO 对其进行短期诱导胁迫，测定了蔊菜的生物量、生理活性物质和主要药用成分。
在碱性盐胁迫下，蔊菜的过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、过氧化物酶（POD）和抗坏血酸过氧化物酶（APX）活性升高，脯氨酸（Pro）和丙二醛（MDA）积累增加。此外，对药用成分的分析表明，在 0.6%NaCO 胁迫 48 h、0.2%NaCO 胁迫 72 h、0.4%NaCO 胁迫 12 h 和 0.4%NaCO 胁迫 8 h 时，蔊菜产生的可溶性糖、黄酮类、熊果酸和齐墩果酸含量最高。
短期 NaCO 胁迫可增强蔊菜药用成分的合成。通过调控胁迫条件，可以优化各种药用物质的产生。这种方法可能为蔊菜的定向栽培提供基础，有望在治疗各种疾病方面得到应用。
