Yuan Chun-ling, Guo Wei-ying
Liaoning Medical College, Jinzhou 121001, China.
Zhong Yao Cai. 2009 Dec;32(12):1894-8.
To study the purification process of total flavones from Glechoma longituba with macroporous adsorption resin.
The eluting power and purified ratio of total flavones were selected as indices, the adsorbility and eluting parameters of the process were studied.
The best result that the purity of total flavones from Glechoma longituba could reach 68. 94% was based on the followings: total flavones content in the liquid-0.383 mg/mL, feeding volume--20 mL, feeding rate--1.0 mL/min, eluting agent-5BV purify water and 10BV 30% ethanol, eluting rate--1.0 mL/min.
The process is simple and convenient which can be used in the purification of total flavones from Glechoma longituba, it may has a good application foreground.
研究用大孔吸附树脂纯化连钱草总黄酮的工艺。
以总黄酮的洗脱能力和纯化率为指标,研究该工艺的吸附性能和洗脱参数。
在以下条件下,连钱草总黄酮纯度可达68.94%,效果最佳:液体中总黄酮含量-0.383mg/mL,上样量-20mL,上样流速-1.0mL/min,洗脱剂-5BV纯化水和10BV 30%乙醇,洗脱流速-1.0mL/min。
该工艺简单便捷,可用于连钱草总黄酮的纯化,具有良好的应用前景。