Department of Animal Science, University of Tennessee Institution of Agriculture, Knoxville, TN, USA.
BMC Res Notes. 2024 Oct 14;17(1):305. doi: 10.1186/s13104-024-06917-2.
Heifer development is crucial for the optimization of reproductive efficiency in beef production. Heifer development is largely influenced by nutrition. Nutritional status of these heifers can influence immunological responses that are crucial for reproduction. Commercial Angus heifers (n = 9) were utilized, in a pilot study, to elucidate the effects of sampling time (days 0, 7, 14, 21, 28, and 35), pregnancy status, and type of mating on individual cytokine concentrations and cytokine profiles in the vagina following breeding.
Cytokine profiles were analyzed using MetaboAnalyst 5.0 and one-way ANOVAs were performed in R Studio to identify differences in individual cytokines based on sampling time, pregnancy status, and type of mating. Cytokine profiles were different (P = 0.05) 7 days after either mating type. Cytokines, IL-1β, IL-17a, MCP-1, and TNFα were different based on the mating type and pregnancy status. Multiple cytokines, IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-17a, VEGFa, and MIP1α, were different between days regardless of pregnancy status. In conclusion, vaginal cytokines differ based on pregnancy status, type of mating, and time which may be indicative of vital pathways that need to be activated for pregnancy.
小母牛的发育对于优化肉牛生产的繁殖效率至关重要。小母牛的发育在很大程度上受到营养的影响。这些小母牛的营养状况可以影响对繁殖至关重要的免疫反应。在一项初步研究中，使用商业安格斯小母牛（n=9）来阐明采样时间（第 0、7、14、21、28 和 35 天）、怀孕状态和配种类型对配种后阴道中个体细胞因子浓度和细胞因子谱的影响。
使用 MetaboAnalyst 5.0 分析细胞因子谱，在 R Studio 中进行单因素方差分析，根据采样时间、怀孕状态和配种类型确定个体细胞因子的差异。两种配种类型后 7 天细胞因子谱不同（P=0.05）。细胞因子 IL-1β、IL-17a、MCP-1 和 TNFα根据配种类型和怀孕状态而不同。多种细胞因子，包括 IL-1α、IL-1β、IL-6、IL-8、IL-10、IL-17a、VEGFa 和 MIP1α，无论怀孕状态如何，在不同天数之间存在差异。总之，阴道细胞因子根据怀孕状态、配种类型和时间而不同，这可能表明需要激活关键途径才能怀孕。
